PMID: 3768395Nov 14, 1986Paper

Liposomes and tear fluid. I. Release of vesicle-entrapped carboxyfluorescein

Biochimica Et Biophysica Acta
R F Barber, P N Shek

Abstract

The successful application of liposomes as a topical ophthalmic drug delivery device requires knowledge of vesicle stability in the presence of tear fluid. The release of 5-carboxyfluorescein from large unilamellar liposomes in the presence of rabbit tear fluid was studied in vitro as a function of bilayer cholesterol content. Reverse evaporation vesicles were prepared from egg phosphatidylcholine, stearylamine and varying amounts of cholesterol. Both the rate and the extent of fluorescent dye release were significantly increased in the presence of rabbit tear fluid at all cholesterol levels. However, by incorporating increasing amounts of cholesterol in the vesicle bilayers, tear-induced leakage was reduced. The release kinetics reported in this study are similar to those observed in the presence of human serum. While serum-induced leakage is attributed to high-density lipoprotein-mediated destabilization, reported differences in tear protein composition suggest some other, as yet unidentified, factor.

References

Oct 18, 1976·Biochemical and Biophysical Research Communications·L KruppP I Brecher
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Jan 26, 1978·Nature·J T DingleJ S Lowe
Mar 1, 1985·Survey of Ophthalmology·V H LeeD J Schanzlin
Mar 1, 1985·Medical Hypotheses·W Westerhof
Jan 1, 1980·Annual Review of Biophysics and Bioengineering·F Szoka, D Papahadjopoulos

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Citations

Dec 31, 2003·International Journal of Pharmaceutics·Indu P KaurDeepika Aggarwal
Mar 8, 2005·Survey of Ophthalmology·Shehab EbrahimPaul J Lee
Aug 15, 2014·Drug Delivery·Renu AgarwalNafeeza Mohd Ismail
Jul 1, 1989·Journal of Microencapsulation·R F BarberN Z Stanacev
May 14, 2009·Seminars in Ophthalmology·Joseph B CiolinoDaniel S Kohane
Apr 19, 2015·Journal of Biomaterials Applications·Myrto KorogiannakiHeather Sheardown
Jun 3, 2021·Pharmaceutics·Jose Navarro-PartidaArturo Santos

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