PMID: 698196Sep 5, 1978Paper

Liposomes from ionic, single-chain amphiphiles

Biochemistry
W R Hargreaves, D W Deamer

Abstract

In studies of the minimum physiochemical requirements for lipid membrane formation, we have made liposomes from dilute, aqueous dispersions of C(8)-C(18) single-chain amphiphiles. In general, membrane formation from ionic soaps and detergents requires the presence of uncharged amphiphiles. Vesicles were characterized by phase-contrast microscopy, by trapping of ionic dyes, as well as by negativestain and freez-frature electron microscopy. They were typically heterogeneous in size, but the average diameter could be experimentally varied in some cases over the range of 1 to 100 micrometer. Uni-, oligo-, and multilamellar vesicles were observed. Membrane permeability to various solutes was determined in part by a new technique which utilized phase-contract microscopy; when impermeable vesciles exclude added solutes such as sucrose, refractive index differences are created between vesicle contents and surrounding medium, so that the vesicles appear bright in the phase microscope. Permeant solutes do not produce this effect. Spectrophotometric permeability determinations confirmed the results of this technique and provided quantitative measures of permeability. Monoalkyl liposomes have potential uses as models of biomembranes and in...Continue Reading

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Citations

Oct 17, 2002·The Anatomical Record·Pierre-Alain Monnard, David W Deamer
Oct 17, 2002·The Anatomical Record·Catherine E Morris
Dec 1, 1994·Journal of Molecular Evolution·A C ChakrabartiD W Deamer
Jan 1, 1982·Journal of Molecular Evolution·D W Deamer, G L Barchfeld
Jan 1, 1988·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·H YanagawaM Ito
Jan 1, 1988·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·H J MorowitzD W Deamer
Jan 1, 1986·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·D W Deamer
Jan 1, 1991·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·I BaezaJ Oró
Oct 29, 2005·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Arthur L Weber
Dec 1, 2006·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Robert M Hazen, David W Deamer
Mar 16, 2007·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Jacquelyn A Thomas, F R Rana
May 29, 2007·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·H H ZepikD Deamer
Sep 18, 2007·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Maya Fishkis
Jun 19, 2008·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Trishool Namani, David W Deamer
Jul 18, 2009·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Tiina Laiterä, Kirsi Lehto
Nov 17, 2010·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Daniel Balleza
Oct 12, 2012·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Pablo Razeto-Barry
Nov 13, 2008·Pharmaceutical Research·Dimitrios G FatourosAnette Müllertz
Dec 1, 1986·Biochimica Et Biophysica Acta·R Bittman, L Blau
Feb 23, 1994·Biochimica Et Biophysica Acta·C SantaellaB Monasse
Jun 1, 1986·Chemistry and Physics of Lipids·L D MayerP R Cullis
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Jan 1, 1980·Bio Systems·D W Deamer, J Oro
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Nov 1, 2005·Journal of Colloid and Interface Science·Johanna BornéAli Khan
Jul 24, 1998·Trends in Biotechnology·D D Lasic
May 29, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W S PetersA Deri Tomos
Aug 10, 2012·Chemical Reviews·Michael Meot-Ner Mautner
Mar 28, 2009·Journal of the American Chemical Society·Glen B ThomasDouglas S English
Jun 17, 2011·The Journal of Physical Chemistry. B·Panfeng LongJingcheng Hao
Dec 13, 2012·The Journal of Physical Chemistry. B·Wenlong XuJingcheng Hao
Sep 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·F CascheraP-A Monnard
Jun 6, 2008·Nature·Sheref S MansyJack W Szostak

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