Liposomes loaded with P. falciparum merozoite-derived proteins are highly immunogenic and produce invasion-inhibiting and anti-toxin antibodies

Journal of Controlled Release : Official Journal of the Controlled Release Society
Wesley L FotoranGerhard Wunderlich

Abstract

The formulation of an effective vaccine against malaria is still a significant challenge and the induction of high anti-parasite antibody titers plus a sustained T cell response is mandatory for the success of such a vaccine. We have developed a nanoliposome-based structure which contains plasma membrane-associated proteins (PfMNP) of Plasmodium falciparum merozoites on its surface. Incorporation of parasite-derived proteins led to a significant increase in the size and dispersity of particles. Immunization of particles in BalbC and C57BL/6 mice led to high anti-MSP119 IgG titers (10(4)) after the first dose and reached a plateau (>10(6)) after the third dose. While very high titers were observed against the C-terminal domain of the vaccine candidate MSP1, only modest titers (≤10(3)) were detected against MSP2. The induced antibodies showed also a strong growth-inhibiting effect in reinvasion assays. In addition, PfMNP immunization generated antibodies which partially blocked the inflammatory response, probably by blocking TLR-induced activation of macrophages by malarial toxins such as GPI anchors. The results underline the potential of nanoliposome-based formulations as anti-malarial vaccines.

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Citations

Jan 15, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·M J Rodrigues-JesusLuís C S Ferreira
Dec 1, 2015·Biophysics Reviews·Ana Maria Sper SimãoPietro Ciancaglini
Aug 14, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Wesley L FotoranGerhard Wunderlich
Dec 12, 2016·ACS Biomaterials Science & Engineering·Wesley L FotoranGerhard Wunderlich

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