Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through Toll-like receptor 2

Clinical and Diagnostic Laboratory Immunology
Tetsuya MatsuguchiYasunobu Yoshikai

Abstract

Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-alpha) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-kappaB activation and TNF-alpha production in RAW264.7, a mouse macrophage cell line, the activity was found to be as follows: protoplast > cell wall > polysaccharide-peptidoglycan complex. Both crude extracts and purified lipoteichoic acids (LTAs) from two Lactobacillus strains, L. casei and L. fermentum, significantly induced TNF-alpha secretion from RAW264.7 cells and splenocytes of C57BL/6, C3H/HeN, and C3H/HeJ mice but not from splenocytes of C57BL/6 TLR2(-/-) mice. Lactobacillus LTA induced activation of c-Jun N-terminal kinase activation in RAW264.7 cells. Furthermore, in HEK293T cells transected with a combination of CD14 and Toll-like receptor 2 (TLR2), NF-kappaB was activated...Continue Reading

References

Jul 1, 1978·Infection and Immunity·F M Collins, P B Carter
Oct 1, 1992·Physiological Reviews·J P Kraehenbuhl, M R Neutra
Dec 1, 1991·Infection and Immunity·S BhakdiW Fischer
Jul 1, 1983·European Journal of Biochemistry·W FischerR Haas
Oct 1, 1995·FEMS Immunology and Medical Microbiology·Y SudaS Kusumoto
Jan 1, 1996·Annual Review of Cell and Developmental Biology·M P Belvin, K V Anderson
Dec 1, 1996·Chemical & Pharmaceutical Bulletin·V M de AmbrosiniG Oliver
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·F L RockJ F Bazan
Feb 17, 1999·The Journal of Experimental Medicine·S T QureshiD Malo
Apr 10, 1999·The Journal of Biological Chemistry·J C ChowF Gusovsky
Jun 11, 1999·The Journal of Biological Chemistry·R SchwandnerC J Kirschning
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MiettinenS Matikainen
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H LiuR M Pope
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·O TakeuchiS Akira
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T MatsuguchiY Yoshikai
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·A OzinskyA Aderem
Feb 7, 2001·The Journal of Experimental Medicine·S MorathT Hartung
Jul 14, 2001·Science·B PulendranJ Banchereau
Aug 25, 2001·Journal of Endotoxin Research·S AkiraT Kaisho
Aug 30, 2001·Nature Immunology·T HorngR Medzhitov

❮ Previous
Next ❯

Citations

Oct 7, 2008·Trends in Immunology·Kan Shida, Masanobu Nanno
Feb 17, 2010·Nature Reviews. Microbiology·Sarah LebeerSigrid C J De Keersmaecker
Jun 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Corinne GrangetteAnnick Mercenier
Apr 14, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jamie H Rosenblum LichtensteinJoseph D Brain
Apr 10, 2010·Microbiology and Immunology·Heeson Chon, Byungryul Choi
May 26, 2009·Applied and Environmental Microbiology·Pilar Fernández de PalenciaPaloma López
Sep 9, 2005·Clinical and Diagnostic Laboratory Immunology·Gabriel VinderolaGabriela Perdigon
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sarah LebeerSigrid C J De Keersmaecker
Sep 28, 2007·Canadian Journal of Microbiology·Christian BleauLucie Lamontagne
May 13, 2006·American Journal of Respiratory Cell and Molecular Biology·Jamie H Rosenblum LichtensteinJoseph D Brain
Jan 1, 2010·Journal of Nutritional Science and Vitaminology·Keisuke TobitaHajime Otani
Jan 1, 2012·Microbial Ecology in Health and Disease·Sampo J Lahtinen
Aug 13, 2013·The British Journal of Nutrition·Susan E PowerGerald F Fitzgerald
Dec 4, 2004·European Journal of Nutrition·Michael de VreseJürgen Schrezenmeir
Aug 24, 2006·Bioscience, Biotechnology, and Biochemistry·Noritoshi TakahashiTadao Saito
Jan 1, 2011·Bioscience and Microflora·Akinobu Kajikawa, Shizunobu Igimi
Jan 23, 2009·Journal of Gastroenterology·Andrea T BorchersM Eric Gershwin
Jan 31, 2015·Pathogens·Fengjuan YangShiyan Qiao
Oct 13, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·I-Chiao LeePeter A Bron
Oct 28, 2011·Trends in Microbiology·Sarah LebeerJos Vanderleyden
Aug 6, 2011·European Journal of Pharmacology·Daniela M RemusPeter A Bron
Dec 19, 2015·Research in Veterinary Science·W M Quinteiro-FilhoS Sharif
Nov 18, 2008·International Immunopharmacology·Young Hee RyuSeung Hyun Han
May 17, 2007·International Journal of Experimental Pathology·J MarcinkiewiczA Chmielarczyk
Jan 6, 2009·Annals of the New York Academy of Sciences·Carlos R BautistaMagdalena Ku

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