Lipoxin A4 interferes with embryo implantation via suppression of epithelial-mesenchymal transition

American Journal of Reproductive Immunology : AJRI
Zhangye XuYinping Huang

Abstract

To test whether lipoxin A4 (LXA4) interferes with embryo implantation via suppression of epithelial-mesenchymal transition (EMT). We developed a mouse model of LXA4 blocking embryo implantation and detected the indicators of EMT to confirm that LXA4 inhibits EMT might be a mechanism of interfering with the embryo implantation. We detected integrin-linked kinase (ILK), N-formylpeptide receptor 2 (FPR2), vascular endothelial growth factor, matrix metalloproteinases (MMPs), Akt, GSK3β, NF-ĸB, twist, vimentin, fibronectin, and β-catenin mRNA expression using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR; localized protein expression using immunohistochemistry and Western blotting assay; MMPs activity assay by gelatin zymography; and the status of implantation in pregnant animals assessed by pontamine blue reaction test. Preimplantation administration of LXA4 resulted in implantation failure. LXA4 has a time- and dose-dependent effect on embryo implantation. Day 0.5 after fertilization is the most effective time to use LXA4 to block embryo implantation. (a) LXA4 reduced endometrial stroma edema; (b) LXA4 inhibited the activity of MMP9 and significantly upregulated the expression of β-catenin, and down...Continue Reading

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Citations

Jun 4, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Małgorzata SzczukoMaciej Ziętek
Apr 28, 2019·International Journal of Molecular Sciences·Hyunkoo KangBuHyun Youn
Mar 12, 2020·Frontiers in Cell and Developmental Biology·Xiaowei ZhouAijun Zhang
Aug 28, 2021·International Journal of Molecular Sciences·Sheng-Dean LuoChing-Shuen Wang
Jan 14, 2022·Cell and Tissue Research·Farnaz OghbaeiKazuhiko Imakawa

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