PMID: 2124949Sep 1, 1990Paper

Lipoxygenase inhibitors suppress intracellular calcium rise induced by ionomycin in rat thymocytes

Cell Calcium
A S GukovskayaV V Bezuglov

Abstract

The lipoxygenase (LO) inhibitors nordihydroguaiaretic acid (NDGA) and 15S-hydroxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-HETE) have been found to suppress the rise in free cytoplasmic Ca2+ concentration [( Ca2+]i) induced by the Ca2+ ionophores ionomycin and A23187 in rat thymocytes. Bromophenacyl bromide (BPB), a phospholipase A2 (PLA2) inhibitor, produced a much weaker inhibitory effect, and indomethacin, a cyclo-oxygenase inhibitor, practically did not influence the [Ca2+]i response to ionomycin. These findings implicate the involvement of LO product(s) in the [Ca2+]i rise triggered by the Ca2+ ionophores. The contribution of the NDGA-sensitive component to the ionomycin-induced [Ca2+]i rise was significant in the ionomycin concentration range of 0.1 nM to 0.1 microM whereas at higher doses of the ionophore it gradually diminished. By contrast, the [Ca2+]i rise induced by exogenous arachidonic acid (AA) or melittin, a PLA2 activator, was not suppressed but potentiated by NDGA. Ionomycin and exogenous AA also elicited opposite changes in thymocyte cytoplasmic pH (pHi): the former elevated the pHi while the latter induced a pronounced acidification of the cytoplasm. This difference in the pHi responses may account for t...Continue Reading

References

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Citations

Nov 1, 1995·Neuroscience and Behavioral Physiology·A S Pivovarov, W Egido-Villareal
Oct 19, 1992·Biochimica Et Biophysica Acta·A S GukovskayaV V Bezuglov
Aug 27, 2009·Toxicon : Official Journal of the International Society on Toxinology·Raoudha Zouari-KessentiniNaziha Marrakchi

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