PMID: 2492416Feb 1, 1989Paper

Lipoxygenase metabolism of polyunsaturated fatty acids in oocytes of the frog Xenopus laevis

Archives of Biochemistry and Biophysics
D J Hawkins, A R Brash

Abstract

Recently, oocytes or eggs of two marine invertebrates have been found to metabolize arachidonic acid to specific monohydroxy products. These studies have prompted our examination of the oocytes of higher organisms. In the present study, oocytes of an amphibian, Xenopus laevis, were examined for their capacity to biosynthesize hydroxyeicosatetraenoic acids (HETEs) and related hydroxy fatty acids. Two hydroxyeicosanoids were formed during incubations of oocyte homogenates with [14C]arachidonic acid; their structures and stereochemistry were determined by high-pressure liquid chromatography, uv spectroscopy, and gas chromatography-mass spectrometry. The compounds were identified as 15(S)- and 12(S)-hydroxyeicosatetraenoic acids. The synthesis of the two HETEs was not blocked by a cyclooxygenase inhibitor, indomethacin (10 microM), or by prior exposure of the oocyte homogenates to carbon monoxide, an inhibitor of cytochrome P450. Furthermore, 12(S)- and 15(S)-hydroperoxyeicosatetraenoic acids were isolated from brief incubations of gel-filtered ammonium sulfate fraction of frog oocyte homogenates; isolation of the hydroperoxide is further support for the existence of 12(S)- and 15(S)-lipoxygenase activities in the oocytes of X. lae...Continue Reading

References

Aug 1, 1986·Prostaglandins, Leukotrienes, and Medicine·L MeijerR W Bryant
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T S AlonsoA M Pechén de D'Angelo
May 15, 1980·Analytical Biochemistry·J M BoeynaemsW C Hubbard
Jan 1, 1982·Differentiation; Research in Biological Diversity·A K Deshpande, S S Koide
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Apr 1, 1995·Pflügers Archiv : European journal of physiology·G CharpentierF Fournier
May 20, 1993·Biochimica Et Biophysica Acta·M MoriT Shimizu
Dec 2, 2014·Progress in Lipid Research·Thomas HornDagmar Heydeck
Dec 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J Fretland, S W Djuric

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