Lipoxygenase metabolites of arachidonic acid and the development of ischaemic cerebral oedema

Neurological Research
R J DempseyM Maley

Abstract

This study examined the changes in cerebral blood flow, water content, and lipoxygenase metabolites (leukotrienes) following bilateral carotid artery occlusion (BCO) and reperfusion in the gerbil. The effect of inhibiting lipoxygenase with nordihydroguaretic acid (NDGA) was also examined. BCO caused cerebral blood flow (measured using H2 clearance) to decline from 23.5 +/- 1.9 to 4.5 +/- 1.9 ml/min/100 gm. Reperfusion increased flow to 27.9 +/- 4 ml/min/100 gm at 10 min, which declined to 13.7 +/- 1.3 ml/min/100 gm at 50 min. Concomitant oedema measurement revealed brain specific gravity decreasing to 1.0402 +/- 0.0014 at 10 min and to 1.0325 +/- 0.0006 at 50 min reperfusion (nonoccluded controls). Leukotriene B4 (LTB4) increased from 26.8 +/- 4.6 to 33.5 +/- 2.1 pg/mg protein 10 min after reperfusion (p less than 0.05), but declined to 21.8 +/- pg/mg protein by 100 min (vs nonischaemic control = 21.3 +/- 2.9 pg/mg protein). Activation of arachidonate metabolism was confirmed by significantly increased 6 keto PGF1 alpha. Pretreatment of the animals with NDGA did not alter CBF, but increased specific gravity above saline-treated controls at 50 min of reperfusion (NDGA = 1.0370 +/- 0.002 vs control = 1.0325 +/- 0.0006, p less tha...Continue Reading

References

Aug 1, 1992·Acta Neurologica Scandinavica·P GaetaniR Rodriguez y Baena
Jul 1, 1989·Stroke; a Journal of Cerebral Circulation·T NakagomiK Takakura
Aug 11, 2001·British Journal of Pharmacology·P CiceriS Nicosia
Sep 1, 1992·Journal of Neurosurgery·C C ChioK L Black
Oct 1, 1987·Acta Neurologica Scandinavica·R RodriguezL Pacchiarini
Apr 1, 1988·Stroke; a Journal of Cerebral Circulation·K AbeK Kogure
Oct 1, 1988·Journal of Neurosurgery·P PaolettiR Rodriguez y Baena

Related Concepts

Metabolic Process, Cellular
Psammomys
Cerebral Blood Flow Imaging
Tang Hsi Ryu Syndrome
Arachidonic Acid
Brain
Lipoxygenase-2
Regional Blood Flow
Lipoxygenase
Leukotriene B4 Biosynthetic Process

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.