Liquid biopsy of fine-needle aspiration supernatant for lung cancer genotyping

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
N GuibertGeoffrey R Oxnard

Abstract

Tumor genotyping is transforming lung cancer care but requires adequate tumor tissue. Advances in minimally invasive biopsy techniques have increased access to difficult-to-access lesions, but often result in smaller samples. With the advent of highly sensitive DNA genotyping methods used for plasma analysis, we hypothesized that these same methods might allow genotyping of free DNA derived from fine needle aspiration supernatant (FNA-S). We studied patients with known or suspected lung cancer undergoing fine needle aspirate (FNA). After spinning the sample for cellblock, the FNA-S (usually discarded) was saved for genotyping. Supernatant cell-free DNA (SN-cfDNA) was extracted and tested by both droplet digital PCR (EGFR, BRAF, KRAS mutations) and highly sensitive amplicon-based next-generation sequencing (NGS). 17 samples were studied, including 11 FNAs from patients with suspected lung cancer and 6 FNAs from patients with lung cancer and acquired drug resistance. Of 6 newly diagnosed adenocarcinomas, 4 had a driver mutations (1 EGFR, 2 KRAS, 1 HER2) found on tissue; all of these could be detected in SN-cfDNA. The EGFR driver mutation was detected in all 5 adenocarcinomas with acquired EGFR resistance and the EGFR T790 M in th...Continue Reading

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Citations

Oct 15, 2018·Cancer Cytopathology·Sinchita Roy-Chowdhuri
Aug 21, 2018·The Journal of Pathology·Shoko VosAnnelien L Bredenoord
Mar 20, 2019·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B HanniganS Roy-Chowdhuri
Sep 5, 2020·Cancer Cytopathology·Paul A VanderLaan, Sinchita Roy-Chowdhuri
Feb 14, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Nicolas GuibertJulien Mazieres
Mar 2, 2021·Frontiers in Medicine·Pasquale PisapiaGiancarlo Troncone
Apr 2, 2021·Cytopathology : Official Journal of the British Society for Clinical Cytology·Pasquale PisapiaGiancarlo Troncone
Jun 14, 2021·Seminars in Diagnostic Pathology·Deepali Jain, Sinchita Roy-Chowdhuri
Jul 11, 2021·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Joseph R HabibJin He
Sep 1, 2021·Critical Reviews in Oncology/hematology·Sasimol UdomrukParunya Chaiyawat
Sep 28, 2021·Expert Review of Molecular Diagnostics·Umberto MalapelleChristian Rolfo
Jan 21, 2019·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Ibiayi Dagogo-JackJochen K Lennerz
Nov 14, 2021·Molecular Diagnosis & Therapy·Susana Olmedillas-LópezDamián García-Olmo

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