Liquid Biopsy of Vitreous Reveals an Abundant Vesicle Population Consistent With the Size and Morphology of Exosomes

Translational Vision Science & Technology
Yuanjun ZhaoJeffrey M Sundstrom

Abstract

To investigate the molecular components of the vitreous in order to better understand retinal physiology and disease. Vitreous was acquired from patients undergoing vitrectomy for macular hole and/or epiretinal membrane, postmortem donors, and C57BL/6J mice. Unbiased proteomic analysis was performed via electrospray ionization tandem mass spectrometry (MS/MS). Gene ontology analysis was performed and results were confirmed with transmission electron microscopy, atomic force microscopy, and nanoparticle tracking analysis (NTA). Proteomic analysis of vitreous obtained prior to vitrectomy identified a total of 1121 unique proteins. Gene ontology analysis revealed that 62.6% of the vitreous proteins were associated with the gene ontology term "extracellular exosome." Ultrastructural analyses, Western blot, and NTA confirmed the presence of an abundant population of vesicles consistent with the size and morphology of exosomes in human vitreous. The concentrations of vitreous vesicles in vitrectomy patients, postmortem donors, and mice were 1.3, 35, and 9 billion/mL, respectively. Overall, these data strongly suggest that information-rich exosomes are a major constituent of the vitreous. The abundance of these vesicles and the presen...Continue Reading

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Citations

Sep 12, 2019·Scientific Reports·Sirisha ThippabhotlaMei He
Feb 23, 2020·Investigative Ophthalmology & Visual Science·Jason MightyStephen Redenti
Sep 2, 2020·Investigative Ophthalmology & Visual Science·Rebekah RobinsonShruti Sharma
Jan 31, 2019·International Journal of Molecular Sciences·Thierry Léveillard, Laurence Klipfel
Jul 9, 2020·Frontiers in Cell and Developmental Biology·Mi ZhouJeffrey M Sundstrom
Oct 3, 2018·Translational Vision Science & Technology·Gabriel VelezVinit B Mahajan
Jul 17, 2020·Frontiers in Cell and Developmental Biology·Mi ZhouJeffrey M Sundstrom
Oct 22, 2020·Stem Cells International·Raffaele NuzziAlessandro Vercelli
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Joana MaiaBruno Costa-Silva
Jan 15, 2021·Scientific Reports·Tiina ÖhmanSirpa Loukovaara
Jan 8, 2021·Experimental Eye Research·Priscilla Sayami AkamineDânia Emi Hamassaki
Jan 7, 2021·International Journal of Nanomedicine·Jia LiuZhuhong Zhang
Jan 30, 2021·Neural Regeneration Research·Inês Dinis Aires, Ana Raquel Santiago
Jun 3, 2021·Journal of Clinical Medicine·Sarah R WeberJeffrey M Sundstrom
Aug 15, 2021·Biotechnology Advances·Saeedreza Zeibi Shirejini, Fatih Inci

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Datasets Mentioned

BETA
GM

Methods Mentioned

BETA
electrophoresis
AFM
biopsy
dissection
biopsies

Software Mentioned

Mascot
Excel
Scaffold
ExoCarta
ExoQuick
Proteome
Nanoscope IIIa
NanoSight

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