Liquid chromatography assay for routine monitoring of cellular ribavirin levels in blood

Antimicrobial Agents and Chemotherapy
Yoichi InoueYukinao Kohda

Abstract

Ribavirin-induced hemolytic anemia is one cause for cessation of combination therapy with alpha interferon 2b and ribavirin for hepatitis C infection. Determining cellular ribavirin levels in blood, including the levels of its phosphorylated metabolites, might be useful for predicting ribavirin-induced anemia, because the metabolites accumulate in erythrocytes. We simplified an assay method developed previously to make it suitable for routine monitoring of cellular ribavirin. Whole blood diluted with a sixfold volume of ice-cold distilled water was subjected to acid phosphatase digestion to convert phosphorylated ribavirin metabolites to free ribavirin. The resulting mixture, spiked with an internal standard, was treated by phenyl boronic acid column extraction, followed by reverse-phase high-performance liquid chromatography analysis. The calibration curve for ribavirin levels in whole blood was linear at concentrations of 5.3 to 1,024 microM (r(2) = 0.9999). Validation coefficients of variation for intra- and interday assays were 2.9 to 5.8% and 4.3 to 8.3%, respectively. We tested this method by monitoring blood ribavirin concentrations in two hepatitis C patients receiving alpha interferon 2b-plus-ribavirin combination ther...Continue Reading

References

Mar 25, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L De FranceschiR Corrocher
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·S CrottyR Andino
Aug 29, 2002·Alimentary Pharmacology & Therapeutics·C H ChangK A Chan
Oct 31, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael W Fried
Apr 2, 2004·Gastroenterology·Mitchell L ShiffmanUNKNOWN Hepatitis C Antiviral Long-Term Treatment Against Cirrhosis Trial Group
Apr 7, 2004·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Masato HommaYukinao Kohda

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Citations

Jul 24, 2010·Analytical and Bioanalytical Chemistry·Daniel M Müller, Katharina M Rentsch
Oct 22, 2008·The Journal of Antimicrobial Chemotherapy·Judit MorelloVincent Soriano
May 5, 2009·Therapeutic Drug Monitoring·Francois-Ludovic SauvagePierre Marquet
Nov 3, 2009·Biological & Pharmaceutical Bulletin·Masato HommaYukinao Kohda
Mar 19, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zeynep Aydoğmuş
Mar 6, 2013·Drug Metabolism and Pharmacokinetics·Yoshiharu SuzukiYukinao Kohda
Apr 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Antonio D'AvolioGiovanni Di Perri
Oct 23, 2014·Journal of Pharmaceutical Sciences·Tsutomu IshiharaTohru Mizushima
Jul 25, 2008·Biomedical Chromatography : BMC·Zhi-Ling ZhouShu-Guang Lin
Oct 1, 2009·Fundamental & Clinical Pharmacology·Françoise Stanke-LabesqueUNKNOWN French Ribavirin Group OPTIRIB
Aug 9, 2011·Thérapie·Caroline SolasUNKNOWN Groupe Suivi Therapeutique Pharmacologique de la Societe Francaise de Pharmacologie et de Therapeutique
Jun 19, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Arianna LoregianGiorgio Palù
Jul 14, 2007·Journal of Pharmaceutical and Biomedical Analysis·M Espinosa BoschC Bosch Ojeda
Sep 12, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenkui LiFrancis L S Tse

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