Liquid chromatography-atmospheric pressure chemical ionisation/mass spectrometry: a rapid and selective method for the quantitative determination of ginkgolides and bilobalide in ginkgo leaf extracts and phytopharmaceuticals

Phytochemical Analysis : PCA
Anette Gemal JensenFabio Soldati

Abstract

In order to evaluate the composition of active constituents in phytopharmaceutical preparations, valid analytical methods are required. For the determination of the active terpene constituents of Ginkgo biloba (the ginkgolides and bilobalide), a liquid chromatography-mass spectrometry (LC-MS) method has been developed using atmospheric pressure chemical ionisation (APCI) in the negative ion mode. This detection mode was found to be much more sensitive and selective compared to UV; indeed the ginkgo terpene trilactones lack strong UV chromophores and flavonoids interfere with their UV detection. LC-APCI/MS detection allowed a considerable reduction in analysis time when compared to LC-UV, because LC resolution was only needed between the pair of isomers ginkgolide B and ginkgolide J. All compounds were selectively detected by single ion monitoring of their specific deprotonated molecules [M-H]-. The samples were directly injected without pre-purification, and a fast gradient was applied, reducing the total time of analysis to 14 min. With this method, the ginkgo terpene trilactones were detected on-line in the picogram range. Several commercial ginkgo preparations on the Swiss market were analysed, and the ginkgolide and bilobal...Continue Reading

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Citations

Jul 17, 2007·Analytical and Bioanalytical Chemistry·Pei ChenJames Harnly
Mar 2, 2010·Analytical and Bioanalytical Chemistry·Daoquan TangXiaoxing Yin
Dec 4, 2003·Journal of the American Society for Mass Spectrometry·Michael Pikulski, Jennifer S Brodbelt
Sep 11, 2002·Journal of Chromatography. a·Teris A van Beek
Jul 5, 2008·Journal of Agricultural and Food Chemistry·Long-Ze LinJames M Harnly
Mar 9, 2010·Bioscience, Biotechnology, and Biochemistry·Yasuhiro KakigiYukihiro Goda
Jul 5, 2005·Bioorganic & Medicinal Chemistry·Teris A van Beek
Jun 27, 2009·Journal of Pharmaceutical and Biomedical Analysis·Daniela Iuliana SoraAndrei Medvedovici
Jun 14, 2017·Biomolecules & Therapeutics·Kyung-Min LeeHyung-Kyoon Choi
Sep 25, 2004·Mass Spectrometry Reviews·Silas G Villas-BôasJens Nielsen
Jul 12, 2002·Journal of Mass Spectrometry : JMS
Aug 22, 2006·Rapid Communications in Mass Spectrometry : RCM·Shujing DingA Gareth Brenton
Feb 16, 2005·Journal of Mass Spectrometry : JMS·Yongkai SunRichard B van Breemen
Sep 30, 2008·Pakistan Journal of Biological Sciences : PJBS·Zahra RamezaniHossein Keyghobadi

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