Liquid chromatography-electrospray ionization mass spectrometry determination of methylphenidate and ritalinic acid in conventional and non-conventional biological matrices

Journal of Pharmaceutical and Biomedical Analysis
Emilia MarcheiSimona Pichini

Abstract

A procedure based on liquid chromatography-electrospray ionization mass spectrometry is described for determination of methylphenidate (MPH) and its principal metabolite ritalinic acid (RA) in plasma, urine, oral fluid and sweat using 3,4-methylendioxypropylamphetamine (MDPA) as internal standard. Aliquots of 100microL biological fluids and sweat patch were initially treated with acetonitrile, centrifuged, and clear supernatants evaporated and redissolved in 10mM ammonium acetate. Chromatography was performed on a reversed-phase column using a gradient of 10mM ammonium acetate and acetonitrile as a mobile phase at a flow rate of 1mL/min. Separated analytes were confirmed and quantified by positive electrospray ionization mass spectrometry and selected ion monitoring acquisition mode. Limits of quantifications were 1ng/mL plasma, 1ng/sweat patch, 0.5ng/mL oral fluid and urine for MHF; 1ng/mL plasma and oral fluid, 1ng/sweat patch, 0.5ng/mL urine for RA using 100microL biological fluids or one sweat-patch per assay. Calibration curves were linear over the calibration ranges for both MPH and RA, with r(2)>0.99. At three concentrations spanning the linear dynamic range of the assay, mean recoveries ranged between 67.9-90.3% for MPH...Continue Reading

References

Aug 1, 1996·Journal of the American Academy of Child and Adolescent Psychiatry·D P Cantwell
Mar 3, 1998·Lancet·J M SwansonD P Cantwell
Jun 5, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·G A Bach, J Henion
Dec 29, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Alvin C BronsteinStuart E Heard

❮ Previous
Next ❯

Citations

Mar 17, 2010·Analytical and Bioanalytical Chemistry·Mitsuhiro WadaKenichiro Nakashima
Mar 5, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A SeçilirR A A Mathôt
Oct 16, 2012·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·E PapaseitM Farré
Jan 26, 2010·Forensic Science International : Synergy·E MarcheiS Pichini
Oct 23, 2019·BMC Chemistry·Haitham AlRabiahGamal Abdel-Hafiz Mostafa
Sep 1, 2017·New Biotechnology·Aza KobakhidzeMonika Čvančarová

❮ Previous
Next ❯

Related Concepts

Related Feeds

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.