Liquid Chromatography-Mass Spectrometry-Based Rapid Secondary-Metabolite Profiling of Marine Pseudoalteromonas sp. M2

Marine Drugs
Woo Jung KimJong Suk Lee

Abstract

The ocean is a rich resource of flora, fauna, and food. A wild-type bacterial strain showing confluent growth on marine agar with antibacterial activity was isolated from marine water, identified using 16S rDNA sequence analysis as Pseudoalteromonas sp., and designated as strain M2. This strain was found to produce various secondary metabolites including quinolone alkaloids. Using high-resolution mass spectrometry (MS) and nuclear magnetic resonance (NMR) analysis, we identified nine secondary metabolites of 4-hydroxy-2-alkylquinoline (pseudane-III, IV, V, VI, VII, VIII, IX, X, and XI). Additionally, this strain produced two novel, closely related compounds, 2-isopentylqunoline-4-one and 2-(2,3-dimetylbutyl)qunoline-4-(1H)-one, which have not been previously reported from marine bacteria. From the metabolites produced by Pseudoalteromonas sp. M2, 2-(2,3-dimethylbutyl)quinolin-4-one, pseudane-VI, and pseudane-VII inhibited melanin synthesis in Melan-A cells by 23.0%, 28.2%, and 42.7%, respectively, wherein pseudane-VII showed the highest inhibition at 8 µg/mL. The results of this study suggest that liquid chromatography (LC)-MS/MS-based metabolite screening effectively improves the efficiency of novel metabolite discovery. Addit...Continue Reading

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Citations

Mar 7, 2017·Journal of Chromatography. a·Nadezhda A Nekrasova, Svetlana V Kurbatova
Aug 25, 2019·Marine Drugs·Shaden A M KhalifaHesham R El-Seedi
Apr 4, 2019·Applied Microbiology and Biotechnology·Noor AkbarNaveed Ahmed Khan
Dec 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mi Eun KimJun Sik Lee
Jan 18, 2018·Natural Product Reports·John W BluntMichèle R Prinsep
Dec 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad SaalimBenjamin R Clark
May 11, 2019·Physics of Life Reviews·Ermenegilda ParrilliRenato Fani

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Datasets Mentioned

BETA
KJ407077

Methods Mentioned

BETA
NMR
PCR

Software Mentioned

Xcalibur
Clustal X
MEGA

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