Liquid chromatography-positive ion electrospray mass spectrometry method for the quantification of citalopram in human plasma

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Constantinos PistosJulia Atta-Politou

Abstract

A rapid, sensitive and novel narrow-bore liquid chromatography-mass spectrometric method was developed and fully validated for the quantification of citalopram in human plasma. The analyte and internal standard (imipramine) were extracted by liquid-liquid extraction with a mixture of hexane-heptane-isopropanol (88:10:2, v/v/v). The use of a Hypersil BDS C(8) micro-bore column (250 mm x 2.1 mm i.d.; 3.5 microm particle size), results in substantial reduction in solvent consumption. The mobile phase consisted of 10 mM ammonium formate-formic acid (pH 4.5) and acetonitrile (30:70, v/v), pumped at a flow rate of 0.15 ml min(-1). The analytes were detected after positive electrospray ionization using the selected ion-monitoring mode of the species at m/z 325 for citalopram and m/z 281 for imipramine. The method had a chromatographic run time of 10.0 min and a linear calibration curve over the range 0.50-250 ng ml(-1) (r(2) > 0.996). The limit of quantitation was 0.50 ng ml(-1). Accuracy and precision were below the acceptance limits of 15%.

References

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Citations

May 24, 2007·Analytical and Bioanalytical Chemistry·Franck Saint-MarcouxPierre Marquet
Apr 10, 2012·Journal of Materials Science. Materials in Medicine·Majid AbdoussZahra Shariatinia
Oct 3, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Romana UřinovskáMilan Grundmann
Feb 2, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tao JiangHongzhuan Chen
Apr 3, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Júlia MenegolaElfrides E S Schapoval
May 16, 2008·Journal of Separation Science·Ramisetti Nageswara RaoRamaram Narsimha
Jul 22, 2005·Journal of Mass Spectrometry : JMS
Aug 20, 2011·Biomedical Chromatography : BMC·Marcello LocatelliFrancesco Epifano

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