Liquid chromatography quadrupole time-of-flight mass spectrometry selective determination of ochratoxin A in wine

Food Chemistry
T Rodríguez-CaboR Cela

Abstract

The performance of liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) for ochratoxin A (OTA) determination in wine is evaluated for the first time. Sample preparation was optimized to obtain quantitative recoveries at the same time that the efficiency of electrospray ionization (ESI) remained unaltered between sample extracts and calibration standards. Under final conditions, samples (20mL) were concentrated using a reversed-phase solid-phase extraction (SPE) cartridge, followed by OTA elution with 1mL of ethyl acetate. The absolute recoveries of the method, established against calibration standards, were 91-121% and 90-113% (without and with internal standard correction, respectively), for wines fortified at 3 concentration levels. The attained LOQ (0.05ngmL(-1)) remained below the maximum permitted OTA concentration (2ngmL(-1)) in dry wines. The method was applied to different samples, with OTA being found in some dessert wines at concentrations below 1ngmL(-1). The ethyl ester of OTA (OTC) could be identified in the same wine samples from its accurate full product ion spectra.

References

Feb 6, 2003·Journal of Agricultural and Food Chemistry·Gordon S ShephardVikash Sewram
Dec 30, 2006·Molecular Nutrition & Food Research·Annie Pfohl-Leszkowicz, Richard A Manderville
Jul 28, 2012·Journal of the Science of Food and Agriculture·Michelle F TerraLuís R Batista
Jan 25, 2013·Journal of Agricultural and Food Chemistry·Jaime Nácher-MestreFélix Hernández
May 16, 2014·Journal of Agricultural and Food Chemistry·Cristina GiovannoliClaudio Baggiani
Jul 22, 2014·Journal of Agricultural and Food Chemistry·Julia Bellver SotoAna Juan-García
Aug 1, 2014·Journal of Agricultural and Food Chemistry·Qi Ding ZhongYong Ning Wu

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Citations

Feb 16, 2021·Critical Reviews in Food Science and Nutrition·Xianjiang LiHongmei Li
Oct 17, 2020·Analytical and Bioanalytical Chemistry·Jingming ZhouAiping Wang

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