Liquid chromatography tandem mass spectrometry method for the quantification of amisulpride with LLOQ of 100 pg/mL using 100 microL of plasma

Biomedical Chromatography : BMC
Ramakrishna NirogiK Mukkanti


A sensitive and selective high-performance liquid chromatography-positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of amisulpride in 100 microL of human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective (M + H)(+) ions, m/z 370-242 for amisulpride and m/z 341-112 for the internal standard. The assay exhibited a linear dynamic range with a lower range of 0.1-100 ng/mL and a higher range of 1-500 ng/mL of amisulpride in human plasma. The lower limit of quantification was 0.1 ng/mL with a relative standard deviation of less than 10%. Acceptable precision and accuracy were obtained for both linearity ranges. A run time of 2.0 min for each sample made it possible to analyze more than 275 human plasma samples per day. The validated method has been successfully used to analyze plasma samples for application in pharmacokinetic studies.


Mar 29, 2001·Journal of Clinical Psychopharmacology·X XiberasM L Paillère-Martinot
Dec 13, 2001·European Journal of Pharmacology·M P CastelliL Pani
Feb 2, 2002·The American Journal of Psychiatry·Stefan LeuchtWerner Kissling
Jul 20, 2002·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·J PeuskensA Puech
Dec 31, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Julia SachseChristoph Hiemke
May 3, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·F PéhourcqB Bégaud
Jun 11, 2003·Archives of General Psychiatry·John M DavisIra D Glick
Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christine FrahnertKatja Grasmäder
Feb 20, 2004·The International Journal of Neuropsychopharmacology·Ann M Mortimer
Apr 2, 2004·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·N BergemannA Frick
Aug 5, 2004·Neuropsychobiology·Giorgio RacagniMario Amore
Nov 4, 2004·CNS Drugs·Kate McKeage, Greg L Plosker
Jun 22, 2006·Current Drug Metabolism·Yunsheng Hsieh, Walter A Korfmacher
Jun 27, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·H Kirchherr, W N Kühn-Velten

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