Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

Clinical Biochemistry
Mark M KushnirA Wayne Meikle

Abstract

Liquid chromatography tandem mass spectrometry is one of the most specific techniques available in clinical laboratories. In the past, immunoassays were the primary methodology for analysis of steroids in biological samples because they are rapid and easy to perform. However, these methods were shown to suffer from the lack of specificity for measuring many of the diagnostically important steroids. LC-MS/MS overcomes many of the limitations of immunoassays, enhances diagnostic utility of the testing, and expands diagnostic capabilities in endocrinology. In addition to the superior quality of the measurements, LC-MS/MS allows high throughput testing using small sample volume with minimal sample preparation, and frees the laboratory from dependence on suppliers of assay specific reagents. LC-MS/MS is being widely employed for routine measurement of steroids, and the methodology plays an important role in the standardization and harmonization of measurements among clinical laboratories.

References

Dec 1, 1979·Clinical Endocrinology·R F WalkerD Riad-Fahmy
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·C R EdwardsJ R Seckl
May 1, 1996·Clinical Endocrinology·P M Stewart
Apr 1, 1994·Nuclear Medicine and Biology·R Ekins
May 19, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P S Green, J W Simpkins
Jul 21, 2000·The Journal of Clinical Endocrinology and Metabolism·M AsunciónH F Escobar-Morreale
Sep 25, 2001·Journal of Applied Physiology·R K Dubey, E K Jackson
Nov 20, 2001·Chemical Reviews·D H Chace
Mar 14, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·G F van RooyenA F Hundt
Jul 16, 2002·Annals of Clinical Biochemistry·A A A IsmailJ H Barth
Aug 6, 2002·The Journal of Clinical Endocrinology and Metabolism·Michael P CaulfieldDelbert A Fisher
Sep 11, 2002·Rapid Communications in Mass Spectrometry : RCM·Russell P GrantShelley Mackenzie-McMurter
May 27, 2003·Clinical Chemistry·Mark M KushnirA Wayne Meikle
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Jul 26, 2003·Clinical Chemistry·David A Herold, Robert L Fitzgerald
Aug 14, 2003·The Journal of Clinical Endocrinology and Metabolism·A Wayne MeikleAlan H Terry
Feb 7, 2004·The Journal of Clinical Endocrinology and Metabolism·Christina WangRonald S Swerdloff
May 18, 2004·Analytical Chemistry·Karl A HanoldJack A Syage
Aug 26, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Helge Erik Solberg
Aug 27, 2004·Journal of Mass Spectrometry : JMS·Gérard HopfgartnerLuc Alexis Leuthold
Mar 16, 2005·Clinical Biochemistry·Mark M KushnirFrancis M Urry
May 18, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Songmei GaoH T Karnes
Jun 9, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu Chen BarrettOle Vesterqvist
Jun 18, 2005·The Proceedings of the Nutrition Society·Claire J StockerMichael A Cawthorne
Sep 7, 2005·Cell·Annette HammesThomas E Willnow
Nov 22, 2005·Clinical Chemistry·Mark M KushnirA Wayne Meikle
Feb 21, 2006·Clinical Endocrinology·Alison M Jones, John W Honour

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Citations

Mar 16, 2013·Reviews in Endocrine & Metabolic Disorders·Phillip J MonaghanPeter J Trainer
Apr 27, 2013·Reviews in Endocrine & Metabolic Disorders·Flaminia FanelliUberto Pagotto
Apr 25, 2012·BMC Research Notes·Hans Peter SöndergaardJonas Bergquist
Sep 10, 2013·PloS One·Anna-Karin LennartssonIngibjörg H Jonsdottir
Nov 30, 2011·Bioanalysis·Lewis CouchmanNorman F Taylor
Dec 24, 2011·Bioanalysis·Pan DengDafang Zhong
Dec 22, 2012·Bioanalysis·Mark M KushnirJonas Bergquist
Apr 1, 2014·Chromatographia·Fidel AllendeCarlos E Fardella
Apr 15, 2014·Journal of Analytical Methods in Chemistry·Sanghoo LeeYoung-Jin Kim
Mar 5, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·T SøeborgA M Andersson
Oct 15, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Mirko PeitzschGraeme Eisenhofer
Dec 18, 2013·Bioanalysis·Mariëtte Theodora Ackermans, Erik Endert
Jan 11, 2014·Asian Journal of Andrology·Hubert W VesperYuesong Wang
Oct 10, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Giorgia AntonelliMario Plebani
Jan 19, 2016·Analytical and Bioanalytical Chemistry·Spiro KhouryPierre Chaminade
Jan 1, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Wei GaoTobias Stalder
Jan 3, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Carla A PiccinatoRui A Ferriani
Jan 3, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Therese KoalManfred Rauh
Sep 13, 2011·Clinics in Laboratory Medicine·Russell P Grant
Jun 21, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Pekka Keski-RahkonenSeppo Auriola
Jan 15, 2016·Journal of Chromatographic Science·Eleni KalogeraAntonia Gounaris
Mar 25, 2016·Life Sciences·Reggiani V GonçalvesFoued S Espindola
Sep 22, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Mark M KushnirJonas Bergquist
Feb 23, 2013·Psychoneuroendocrinology·Anna-Karin LennartssonIngibjörg H Jonsdottir
Jan 7, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Giorgia AntonelliMario Plebani
Apr 15, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Liezl M BloemAmanda C Swart
Jan 22, 2013·Molecular and Cellular Endocrinology·Kerry M Wooding, Richard J Auchus
Oct 18, 2011·Chemistry Central Journal·Nawed Ik DeshmukhDeclan P Naughton

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