Liquid chromatography with amperometric detection for the determination of non-steroidal anti-inflammatory drugs in plasma

Journal of Chromatography
A G Kazemifard, D E Moore

Abstract

A simple and sensitive liquid chromatographic method with electrochemical detection is described for the quantitative determination of the non-steroidal anti-inflammatory drugs diflunisal, indomethacin, naproxen, piroxicam and sulindac in human plasma. Isolation of the drug from the biological fluid is achieved using a Sep-pak RP18 cartridge. Separation of plasma components occurs on a reversed-phase C18 column with a mobile phase consisting of methanol-water-phosphate buffer. For the amperometric detection the potential of +0.9 V was set on the working electrode. The detection limit of the assay is 10-20 ng/ml. The method showed good concordance for plasma samples containing the drugs (r = 0.999) and can be readily utilized for clinical pharmacokinetic studies.

References

Feb 11, 1979·Journal of Chromatography·D Pitrè, M Grandi
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Citations

Oct 1, 1993·Journal of Pharmaceutical and Biomedical Analysis·C Sánchez-PedreñoJ Rodriguez
Dec 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·G F RayS C Wheeler
May 31, 2001·Journal of Chromatography. a·M CakrtI Skacáni
Jul 20, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·A BakkaliF Vicente
Mar 29, 2001·Journal of Pharmaceutical and Biomedical Analysis·F Sayin, S Kir
Nov 13, 2012·Journal of Chromatographic Science·Dinesh S PatelMallika Sanyal
Jun 1, 1995·The Journal of Pharmacy and Pharmacology·C Hoyo-VadilloF J López-Munoz
Feb 1, 1997·Redox Report : Communications in Free Radical Research·D E Moore, B C Chen
Mar 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·L I Bebawy, N M El-Kousy
Sep 15, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Koichi SuenamiSusumu Kanno
Feb 20, 2007·Journal of Separation Science·Koichi SuenamiSusumu Kanno
May 31, 2019·Journal of Analytical Methods in Chemistry·Hassan A M HendawyElmorsy Khaled

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