Liquid-liquid extraction for recovery of paclitaxel from plant cell culture: solvent evaluation and use of extractants for partitioning and selectivity.

Biotechnology Progress
Timothy J McPartlandSusan C Roberts

Abstract

A major challenge in the production of metabolites by plant cells is the separation and purification of a desired product from a number of impurities. An important application of plant cell culture is the biosynthesis of the anticancer agent paclitaxel. Liquid-liquid extraction plays a critical role in the recovery of paclitaxel and other valuable plant-derived products from culture broth. In this study, the extraction of paclitaxel and a major unwanted by-product, cephalomannine, from plant cell culture broth into organic solvents is quantified. Potential solvent mixtures show varying affinity and selectivity for paclitaxel over cephalomannine. The partition coefficient of paclitaxel is highest in ethyl acetate and dichloromethane, with measured values of 28 and 25, respectively; however, selectivity coefficients are less than 1 for paclitaxel over cephalomannine for both solvents. Selectivity coefficient increases to 1.7 with extraction in n-hexane, but the partition coefficient decreases to 1.9. Altering the pH of the aqueous phase results in an increase in both recovery and selectivity using n-hexane but does not change the results for other solvents significantly. The addition of extractants trioctylamine (TOA) or tributyl...Continue Reading

References

Oct 1, 1994·Journal of Pharmaceutical Sciences·S V BalasubramanianR M Straubinger
Mar 30, 2001·European Journal of Biochemistry·K PaálL Hegedûs
May 27, 2004·Biotechnology and Bioengineering·Michael C Naill, Susan C Roberts
Apr 9, 2008·Molecular Pharmaceutics·Martin E KoleweSusan C Roberts
Dec 20, 1987·Biotechnology and Bioengineering·Z Likidis, K Schügerl
Aug 24, 2010·Biotechnology Progress·Oscar ExpositoRosa M Cusido

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Citations

Sep 29, 2015·Current Biology : CB·Sameh S M SolimanManish N Raizada
Apr 30, 2015·Biotechnology Advances·J F BuyelR Fischer
Sep 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhijian TanFenfang Li
Aug 17, 2019·Frontiers in Bioengineering and Biotechnology·Ljubomir GrozdevSonja Berensmeier

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