Liquid-liquid extraction of commercial and biosynthesized nisin by aqueous two-phase micellar systems

Enzyme and Microbial Technology
Angela Faustino JozalaAdalberto Pessoa

Abstract

Nisin is a natural additive for conservation of food, and can also be used as a therapeutic agent. Nisin inhibits the outgrowth of spores, the growth of a variety of Gram-positive and Gram-negative bacteria. In this paper we present a potentially scalable and cost-effective way to purify commercial and biosynthesized in bioreactor nisin, including simultaneously removal of impurities and contaminants, increasing nisin activity. Aqueous two-phase micellar systems (ATPMS) are considered promising for bioseparation and purification purposes. Triton X-114 was chosen as the as phase-forming surfactant because it is relatively mild to proteins and it also forms two coexisting phases within a convenient temperature range. Nisin activity was determined by the agar diffusion assay utilizing Lactobacillus sake as a sensitive indicator microorganism. Results indicated that nisin partitions preferentially to the micelle rich-phase, despite the surfactant concentration tested, and its antimicrobial activity increases. The successful implementation of this peptide partitioning, from a suspension containing other compounds, represents an important step towards developing a separation method for nisin, and more generally, for other biomolecule...Continue Reading

References

Aug 1, 1966·Journal of General Microbiology·A Hurst
Apr 1, 1993·Journal of Biotechnology·G C TerstappenM R Kula
Jan 1, 1994·Critical Reviews in Food Science and Nutrition·J N Hansen
Feb 1, 1996·Antonie van Leeuwenhoek·J Delves-BroughtonJ Hugenholtz
Jul 1, 1995·Emerging Infectious Diseases·A Dubois
Jan 1, 1997·International Journal of Food Microbiology·V Carolissen-MackayJ W Hastings
Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·C L LiuD Blankschtein
Dec 9, 1993·Journal of Aging & Social Policy·S O Daatland
Nov 26, 1999·Applied Microbiology and Biotechnology·E Parente, A Ricciardi
Oct 11, 2001·Biotechnology and Applied Biochemistry·S H Flôres, R M Alegre
May 23, 2002·Applied Biochemistry and Biotechnology·Thereza Christina Vessoni Penna, Dante Augusto Moraes
Feb 1, 1951·Journal of General Microbiology·A HIRSCH
Jan 12, 2005·Biotechnology and Bioengineering·Henry LamDaniel Blankschtein
May 28, 2005·Applied Biochemistry and Biotechnology·Thereza Christina Vessoni PennaOlivia Cholewa
May 27, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jorge BenavidesMarco Rito-Palomares
Aug 19, 2006·Applied Biochemistry and Biotechnology·Thereza Christina Vessoni PennaOlivia Cholewa
Sep 9, 2006·Applied Biochemistry and Biotechnology·María Elena Mondragón-ParadaEliseo Cristiani-Urbina
Jun 20, 2008·Applied Biochemistry and Biotechnology·Chuanbin LiuShulin Chen

❮ Previous
Next ❯

Citations

Oct 3, 2009·Applied Microbiology and Biotechnology·Tao PanHanshi Qi
Feb 9, 2016·Preparative Biochemistry & Biotechnology·Voltaire Sant'AnnaAdriano Brandelli
Aug 30, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun YuanQirong Shen
Jun 24, 2010·Biotechnology Progress·André M LopesAdalberto Pessoa
Sep 12, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jack C SlootwegDirk T S Rijkers
Feb 28, 2014·Biotechnology Progress·Luciana P MalpiediAdalberto Pessoa
May 5, 2010·Enzyme and Microbial Technology·Zhilong Wang, Zewen Dai
Aug 2, 2011·Bone·Lindsay A BonsignoreEdward M Greenfield
May 21, 2009·Analytical Biochemistry·Luis E González de la Vara, Bárbara Lino Alfaro
May 11, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Marc S HarperBryan Delaney
Jun 14, 2017·Critical Reviews in Food Science and Nutrition·Norfariza JamaluddinFadzlie Wong Faizal Wong
Sep 26, 2019·Frontiers in Bioengineering and Biotechnology·Philippe Gabant, Juan Borrero
Apr 30, 2021·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Hamidreza ParsimehrSeeram Ramakrishna

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.