PMID: 3752985Aug 1, 1986Paper

Liquid-liquid extraction of lactate dehydrogenase from muscle using polymer-bound triazine dyes

Applied Biochemistry and Biotechnology
G Johansson, M Joelsson

Abstract

An extract from porcine muscle containing soluble enzymes has been partitioned between the two liquid phases of an aqueous, biphasic system consisting of dextran, polyethylene glycol, and water. The influence of polymer-bound triazine dyes (Cibacron blue F3G-A and Procion yellow HE-3G) on the partition of lactate dehydrogenase and total protein was studied. The effects of pH and concentrations of polymers and buffer on this so-called affinity partitioning were also determined. The two-phase systems were used in extraction procedures for purification of lactate dehydrogenase to a specific activity of 456-494 U (7.6-8.4 mukat) per mg protein. The use of these systems for extraction of enzymes in technical scale is discussed.

References

Oct 1, 1979·Journal of Chromatography·P D Dean, D H Watson
Jan 1, 1975·Methods in Enzymology·R K Scopes
Dec 23, 1974·Biochemical and Biophysical Research Communications·V P Shanbhag, G Johansson
Mar 1, 1973·European Journal of Biochemistry·G JohanssonP A Albertsson
Jan 1, 1984·Methods in Enzymology·C R Lowe, J C Pearson
Apr 5, 1983·European Journal of Biochemistry·G JohanssonP A Albertsson
Jan 1, 1984·Methods in Enzymology·G Johansson
May 1, 1985·Biotechnology and Bioengineering·G Johansson, M Joelsson

❮ Previous
Next ❯

Citations

Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·G Kopperschläger, J Kirchberger
Oct 18, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yan XuAdalberto Pessoa
Apr 2, 2002·Protein Expression and Purification·Sheryl FernandesRajni Hatti-Kaul
Mar 3, 2010·The Journal of Chemical Physics·Bogdan M LeuWolfgang Sturhahn
Nov 1, 1987·Biotechnology and Bioengineering·F TjerneldM Joelsson
Aug 5, 1989·Biotechnology and Bioengineering·J N BaskirU W Suter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.