Liquid-liquid phase separation and static light scattering of concentrated ternary mixtures of bovine alpha and gammaB crystallins

The Journal of Chemical Physics
George M Thurston

Abstract

We have used light scattering, turbidimetry, and thermodynamic analysis to study the phase diagram of concentrated aqueous mixtures of the bovine lens proteins, gammaB crystallin, and alpha crystallin. We find that dilute alpha crystallin raises the phase separation temperature of concentrated gammaB crystallin, while more concentrated alpha crystallin suppresses phase separation. Very concentrated alpha/gammaB mixtures can reversibly cloud above 37 degrees C, even though gammaB alone phase separates only below temperatures near 0 degrees C, and alpha does not phase separate. At the scattering vector magnitude used, high-concentration alpha/gammaB mixtures scatter less light than the weighted average of their component alpha and gammaB solutions, while low-concentration alpha/gammaB mixtures scatter more than such a weighted average. We use a mean-field thermodynamic analysis of such ternary mixtures to show that the observed light scattering and phase boundaries of alpha and gammaB crystallin mixtures give evidence for prominent local fluctuations of relative protein composition. In the single phase, these fluctuations scatter comparatively little light, but are associated with enhanced thermodynamic instability. By applying t...Continue Reading

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Citations

Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Priya R BanerjeeJayanti Pande
Dec 7, 2007·The Journal of Chemical Physics·A GiannopoulouS N Yannopoulos
Oct 5, 2013·The Journal of Chemical Physics·Chris W WahleGeorge M Thurston
Jul 27, 2012·The Journal of Chemical Physics·Chris W WahleGeorge M Thurston
Jul 27, 2012·The Journal of Chemical Physics·Chris W WahleGeorge M Thurston
Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·George M Thurston
Oct 28, 2015·The Journal of Physical Chemistry Letters·Saskia BucciarelliAnna Stradner
Apr 15, 2016·The Journal of Physical Chemistry Letters·Saskia BucciarelliAnna Stradner
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Giuseppe FoffiPeter Schurtenberger
Aug 3, 2018·Small·Tommy Garting, Anna Stradner
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Feb 10, 2017·The Journal of Chemical Physics·Michael M BellGeorge M Thurston
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