Liquid nitrogen freezers: a potential source of microbial contamination of hematopoietic stem cell components

Transfusion
D FountainR J Benjamin

Abstract

The recent report of hepatitis B transmission between hematopoietic progenitor and putative stem cell (HPC) components stored in liquid nitrogen led to the questioning of whether evidence existed for similar contamination by bacterial or fungal elements. Microbial contamination rates were reviewed for 704 HPC components from 255 patients over an 18-month period. Five liquid nitrogen freezers were surveyed for microbial contamination. The literature was reviewed to ascertain the published experience of other laboratories with HPC component contamination first documented on thawing. Seven (1.2%) of 583 thawed components were found to be contaminated with a variety of environmental or waterborne organisms, despite a meticulous protocol to prevent contamination during thawing. All of these components had been sterile on cryopreservation. Literature review revealed a similar incidence of post-thaw contamination from other centers. Microbial survey of liquid nitrogen freezers revealed low-level contamination in four of five. The organisms represented were similar to those cultured from thawed HPC components. One freezer was heavily contaminated by Aspergillus species. Liquid nitrogen freezers are not sterile, and both the liquid and ...Continue Reading

Citations

May 20, 2009·Cell and Tissue Banking·R VillalbaJ L Gómez Villagrán
Sep 10, 2008·Molecular Biotechnology·John G Day, Glyn N Stacey
Oct 31, 2013·Trends in Biotechnology·P LoiG Ptak
Jun 14, 2000·Animal Reproduction Science·G Vajta
Nov 22, 2002·Biology of Reproduction·Juergen LiebermannMichael J Tucker
Sep 18, 2009·Reproductive Biology and Endocrinology : RB&E·Amr A KaderAshok Agarwal
Jul 9, 2011·PloS One·L Gabriel Sanchez-PartidaJustin C St John
Apr 30, 2014·BioMed Research International·János KoncSándor Cseh
Jan 15, 2014·Tissue Engineering. Part C, Methods·Isobel MassieG John Morris
Nov 1, 2011·Stem Cell Reviews and Reports·Maneesha S InamdarGlyn Stacey
Feb 9, 2008·Critical Reviews in Microbiology·Augusto PessinaLaura Gribaldo
Feb 18, 2016·Biopreservation and Biobanking·Phillip M Baird, Elaine W Gunter
Feb 15, 2002·Human Fertility : Journal of the British Fertility Society·Sue M. AveryKarin J. Dawson
Jan 18, 2012·Expert Opinion on Drug Metabolism & Toxicology·Francesca PistollatoGlyn Stacey
Jan 24, 2013·Expert Opinion on Biological Therapy·Sreedhar ThirumalaErik J Woods
Oct 30, 2014·Systems Biology in Reproductive Medicine·Rakesh SharmaAshok Agarwal
Jan 10, 2012·Reproductive Biomedicine Online·Dominic StoopPaul Devroey
Feb 4, 2006·Reproductive Biomedicine Online·Mark G LarmanDavid K Gardner
Jun 24, 2006·Reproductive Biomedicine Online·Gábor Vajta, Zsolt Péter Nagy
Dec 10, 2002·Reproductive Biomedicine Online·Juergen LiebermannMichael J Levy
Aug 31, 2004·Reproductive Biomedicine Online·D Mortimer
Feb 9, 2010·Fertility and Sterility·Ana CoboJosé Remohí
Feb 14, 2009·Theriogenology·B W W Grout, G J Morris
Sep 13, 2015·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Juan Manuel Bello-LópezJulieta Rojo-Medina
Oct 2, 2004·Andrologia·R LevyB Pozzetto
Oct 29, 2008·Reproduction in Domestic Animals = Zuchthygiene·R E GreenS D Bicudo
Feb 3, 2007·American Journal of Hematology·David BerzPeter J Quesenberry
Jun 15, 2013·International Journal of Laboratory Hematology·A P PatelB S Patel

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