PMID: 5903338Jan 21, 1966

Liquid water in frozen tissue: study by nuclear magnetic resonance

M V Sussman, L Chin


Nuclear magnetic resonance (NMR) spectroscopy was used to examine the behavior and extent of liquid water in postrigor-frozen tissue of cod at temperatures below 0 degrees C. A liquid-water phase persists in the tissue down to about -70 degrees C; the extent of the phase decreases rapidly between 0 degrees and -10 degrees C and slowly at lower temperatures. That the NMR absorption peak of the liquid water increases in width, with decreasing temperature, suggests loss of mobility or structuring of the phase. A technique for introducing geometrically uniform cores of muscle into the probe of the high-resolution spectrometer permits quantitative determinations of liquid water.


Jul 1, 1974·Plant Physiology·M F GeorgeC J Weiser
Apr 1, 1982·Cryobiology·M E Apgar, H O Hultin
Feb 1, 1967·Archives of Biochemistry and Biophysics·N H Grant, H E Alburn
Jul 1, 1971·Journal of Theoretical Biology·P M Wiggins
Oct 8, 1966·Nature·N H Grant, H E Alburn
Jan 1, 1971·Progress in Biophysics and Molecular Biology·J A Walter, A B Hope

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