Liquiritigenin Inhibits Colorectal Cancer Proliferation, Invasion, and Epithelial-to-Mesenchymal Transition by Decreasing Expression of Runt-Related Transcription Factor 2.

Oncology Research
Fan-Chun Meng, Jun-Kai Lin

Abstract

Inhibition of tumor metastasis is one of the most important purposes in colorectal cancer (CRC) treatment. This study aimed to explore the effects of liquiritigenin, a flavonoid extracted from the roots of Glycyrrhiza uralensis Fisch, on HCT116 cell proliferation, invasion, and epithelial-to-mesenchymal transition (EMT). We found that liquiritigenin significantly inhibited HCT116 cell proliferation, invasion, and the EMT process, but had no influence on cell apoptosis. Moreover, liquiritigenin remarkably reduced the expression of runt-related transcription factor 2 (Runx2) in HCT116 cells. Overexpression of Runx2 obviously reversed the liquiritigenin-induced invasion and EMT inhibition. Furthermore, liquiritigenin inactivated the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway in HCT116 cells. Upregulation of Runx2 reversed the liquiritigenin-induced PI3K/AKT pathway inactivation. In conclusion, our research verified that liquiritigenin exerted significant inhibitory effects on CRC invasion and EMT process by downregulating the expression of Runx2 and inactivating the PI3K/AKT signaling pathway. Liquiritigenin could be an effective therapeutic and preventative medicine for CRC treatment.

References

Jun 13, 1992·Lancet·I R Hart, A Saini
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 4, 2006·The Journal of Biological Chemistry·Philip Y WaiPaul C Kuo
Mar 1, 2007·Planta medica·Yong-Wook ShinDong-Hyun Kim
Aug 2, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·René KupferUwe Christians
Dec 9, 2008·The Journal of Surgical Research·Philippe ZerbibBrigitte Jude
Dec 3, 2009·Phytotherapy Research : PTR·Ming ZhouYunqing Cai
Mar 8, 2012·International Journal of Cancer. Journal International Du Cancer·Tomohiko SaseHironobu Sasano
May 30, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dong-Feng NiuRyohei Katoh
Jun 14, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuxin LiuYunqing Cai
Jul 25, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ji-Yeon YuSeikwan Oh

❮ Previous
Next ❯

Citations

Jun 12, 2020·Cancers·Alena LiskovaPeter Kubatka
Jun 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shi WuJinhao Zeng
Nov 28, 2020·Journal of Ethnopharmacology·Zhixin ZhangYing Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
Protein Assay
electrophoresis

Software Mentioned

Rad Image [UNK]
GraphPad

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.