Listeria hijacks host mitophagy through a novel mitophagy receptor to evade killing

Nature Immunology
Yifan ZhangYoucun Qian

Abstract

Cells use mitophagy to remove damaged or unwanted mitochondria to maintain homeostasis. Here we report that the intracellular bacterial pathogen Listeria monocytogenes exploits host mitophagy to evade killing. We found that L. monocytogenes induced mitophagy in macrophages through the virulence factor listeriolysin O (LLO). We discovered that NLRX1, the only Nod-like receptor (NLR) family member with a mitochondrial targeting sequence, contains an LC3-interacting region (LIR) and directly associated with LC3 through the LIR. NLRX1 and its LIR motif were essential for L. monocytogenes-induced mitophagy. NLRX1 deficiency and use of a mitophagy inhibitor both increased mitochondrial production of reactive oxygen species and thereby suppressed the survival of L. monocytogenes. Mechanistically, L. monocytogenes and LLO induced oligomerization of NLRX1 to promote binding of its LIR motif to LC3 for induction of mitophagy. Our study identifies NLRX1 as a novel mitophagy receptor and discovers a previously unappreciated strategy used by pathogens to hijack a host cell homeostasis system for their survival.

References

Dec 10, 2003·The Journal of Cell Biology·Lorraine D HernandezJorge E Galán
Oct 19, 2007·Nature·Amit P BhavsarB Brett Finlay
Jan 18, 2008·Nature·Chris B MooreJenny P-Y Ting
Mar 25, 2010·European Journal of Immunology·Sarah KimVeit Hornung
Dec 3, 2010·Nature·Rongbin ZhouJürg Tschopp
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·Richard J Youle, Derek P Narendra
Jan 12, 2011·The Journal of Cell Biology·Eisuke Itakura, Noboru Mizushima
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Fabrizia StavruPascale Cossart
May 21, 2011·Nature Reviews. Immunology·A Phillip WestSankar Ghosh
Jun 2, 2012·Trends in Microbiology·Mélanie Anne HamonPascale Cossart
Jun 30, 2012·Cell Death and Differentiation·G Ashrafi, T L Schwarz
Jul 6, 2012·Nature·Paras K AnandThirumala-Devi Kanneganti
Dec 6, 2012·Innate Immunity·Fraser SoaresStephen E Girardin
Nov 5, 2013·Neurobiology of Disease·Rubén Gómez-SánchezAnthony H V Schapira
Mar 5, 2014·Autophagy·Ioanna KalvariVasilis J Promponas
Apr 18, 2014·Nature·Mark A CzuczmanJohn H Brumell
May 31, 2014·Nature Cell Biology·Alexandra StolzIvan Dikic
Aug 2, 2014·The Journal of Clinical Investigation·Kenji MizumuraAugustine M K Choi
Jan 7, 2015·The Journal of Clinical Investigation·Marta BuenoAna L Mora
Jun 5, 2015·Nature·Aliaksandr KhaminetsIvan Dikic
Aug 13, 2015·Nature·Michael LazarouRichard J Youle
Oct 27, 2015·Cell·Gerald S Shadel, Tamas L Horvath
Jan 17, 2017·Trends in Microbiology·Heleen Van Acker, Tom Coenye
Feb 21, 2018·Current Biology : CB·Sarah PicklesRichard J Youle
Jul 7, 2018·Cell Calcium·Neeharika NemaniMuniswamy Madesh
Aug 30, 2018·Nature Cell Biology·Konstantinos PalikarasNektarios Tavernarakis

❮ Previous
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Citations

Aug 28, 2019·Cells·Alf Håkon Lystad, Anne Simonsen
Jan 24, 2020·Antioxidants & Redox Signaling·Lei LiuQuan Chen
Jan 4, 2020·Cell Death and Differentiation·Matthew D KellerKen Cadwell
Dec 25, 2019·The Journal of Clinical Investigation·Xiaobo LuoYu L Lei
Mar 3, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Derek M McKayTimothy Shutt
Feb 6, 2020·Veterinary Sciences·Sabari Nath Neerukonda, Upendra Katneni
Sep 15, 2020·The Journal of Cell Biology·Samuel A KillackeyStephen E Girardin
Jun 19, 2019·Oxidative Medicine and Cellular Longevity·Enza VernucciMatteo A Russo
Oct 23, 2019·Signal Transduction and Targeted Therapy·Pei-Pei LiuJian-Sheng Kang
Apr 11, 2020·Frontiers in Cell and Developmental Biology·Guo ChenOliver Kepp
Jun 27, 2020·Frontiers in Cell and Developmental Biology·Valentina SoraElena Papaleo
Nov 12, 2019·Frontiers in Immunology·Elodie RamondAlain Charbit
Nov 5, 2019·Frontiers in Immunology·Margaret A Nagai-SingerIrving C Allen
Jul 17, 2020·Frontiers in Cell and Developmental Biology·Kaili MaQuan Chen
May 29, 2020·Journal of Cell Science·Yingying CongFulvio Reggiori
Sep 29, 2020·International Journal of Chronic Obstructive Pulmonary Disease·Jian-Yu LiuYi-Qing Qu
Mar 27, 2020·Cardiovascular Drugs and Therapy·Tingjuan NiHangyuan Guo
Jul 7, 2020·Autophagy·Julia Sanchez-Garrido, Avinash R Shenoy
Jun 27, 2020·Frontiers in Cell and Developmental Biology·Yigang WangCong Yi
Jul 1, 2020·Frontiers in Endocrinology·Rong YuJian Zhao
Nov 27, 2020·Cell Communication and Signaling : CCS·Yinjuan SongXiangmei Zhou
Dec 15, 2020·Immunology·Robert J Pickering, Lee M Booty
Jul 17, 2019·Journal of Molecular Biology·Terje Johansen, Trond Lamark
Feb 3, 2021·International Journal of Molecular Sciences·Tünde FeketeKitti Pázmándi
Dec 22, 2020·Frontiers in Cellular and Infection Microbiology·Tiia Snäkä, Nicolas Fasel
Feb 20, 2021·Journal of Cellular and Molecular Medicine·Xiaobo LuoQianming Chen
Apr 3, 2020·Current Opinion in Cell Biology·Julia Noack, Shaeri Mukherjee
Mar 7, 2021·Antioxidants·Marc Herb, Michael Schramm
Aug 23, 2020·Signal Transduction and Targeted Therapy·Pei-Pei LiuJian-Sheng Kang
Mar 18, 2021·The FEBS Journal·Andrea Gubas, Ivan Dikic
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Bowen HuXiquan Zhang
May 2, 2021·Clinical and Translational Medicine·Zheng TangNitao Cheng
Jun 7, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Shaoyong ZhuangXu Wang
Jul 18, 2021·Experimental & Molecular Medicine·Yu SongCao Yang

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Methods Mentioned

BETA
electron microscopy
ubiquitination
pull down
pulldown
flow cytometry
electrophoresis
lavage
ELISA
PMA
PCR

Software Mentioned

iLIR
Excel
GraphPad Prism

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