Listeria monocytogenes InlP interacts with afadin and facilitates basement membrane crossing

PLoS Pathogens
Cristina FarallaAnna I Bakardjiev

Abstract

During pregnancy, the placenta protects the fetus against the maternal immune response, as well as bacterial and viral pathogens. Bacterial pathogens that have evolved specific mechanisms of breaching this barrier, such as Listeria monocytogenes, present a unique opportunity for learning how the placenta carries out its protective function. We previously identified the L. monocytogenes protein Internalin P (InlP) as a secreted virulence factor critical for placental infection. Here, we show that InlP, but not the highly similar L. monocytogenes internalin Lmo2027, binds to human afadin (encoded by AF-6), a protein associated with cell-cell junctions. A crystal structure of InlP reveals several unique features, including an extended leucine-rich repeat (LRR) domain with a distinctive Ca2+-binding site. Despite afadin's involvement in the formation of cell-cell junctions, MDCK epithelial cells expressing InlP displayed a decrease in the magnitude of the traction stresses they could exert on deformable substrates, similar to the decrease in traction exhibited by AF-6 knock-out MDCK cells. L. monocytogenes ΔinlP mutants were deficient in their ability to form actin-rich protrusions from the basal face of polarized epithelial monola...Continue Reading

References

Jun 22, 1999·Infection and Immunity·D A Drevets
Sep 24, 1999·The Journal of Cell Biology·J R RobbinsJ A Theriot
May 10, 2000·Nature Medicine·I Guleria, J W Pollard
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
Dec 26, 2001·Current Opinion in Structural Biology·B Kobe, A V Kajava
Dec 23, 2003·Infection and Immunity·Anna I BakardjievDaniel A Portnoy
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
May 5, 2005·The Journal of Infectious Diseases·Anna I BakardjievDaniel A Portnoy
Feb 1, 2006·PLoS Pathogens·Mickey PentecostManuel R Amieva
Mar 15, 2006·Current Opinion in Microbiology·Steffen Backert, Thomas F Meyer
Jul 19, 2006·PLoS Pathogens·Anna I BakardjievDaniel A Portnoy
Oct 13, 2006·Cell Calcium·Kirill KiselyovShmuel Muallem
Nov 23, 2006·Infection and Immunity·Alban Le MonnierSamer Kayal
Apr 20, 2007·Cellular Microbiology·Christelle M RouxRenée M Tsolis
Aug 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juan C Del AlamoJuan C Lasheras
Oct 16, 2007·Clinical Microbiology Reviews·Bryan CoburnB Brett Finlay
Jun 30, 2009·The Journal of Allergy and Clinical Immunology·Katherine R Groschwitz, Simon P Hogan
Oct 29, 2009·Infectious Disorders Drug Targets·W F Anderson
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Edith GouinPascale Cossart
Oct 29, 2010·American Journal of Physiology. Renal Physiology·Nicholas FerrellWilliam H Fissell
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Jun 10, 2011·Journal of Cell Science·Miki Tanaka-OkamotoJun Miyoshi
Nov 16, 2011·Infection and Immunity·Jennifer R RobbinsAnna I Bakardjiev
Dec 16, 2011·Current Opinion in Microbiology·Jennifer R Robbins, Anna I Bakardjiev
May 18, 2012·Molecular Biology of the Cell·Nariaki IwasawaIzumi Oinuma
Aug 23, 2012·PLoS Pathogens·Varvara B Zeldovich, Anna I Bakardjiev
Mar 14, 2013·Progress in Molecular Biology and Translational Science·Kenji MandaiYoshimi Takai
Jul 23, 2013·Biophysical Journal·María Del Rocío Cantero, Horacio F Cantiello
Dec 19, 2013·PLoS Pathogens·Varvara B ZeldovichAnna I Bakardjiev
Mar 19, 2014·The Journal of Cell Biology·Effie BastounisJuan C Lasheras
Dec 3, 2014·Journal of Molecular and Cellular Cardiology·Indroneal BanerjeeVishal Nigam

❮ Previous
Next ❯

Citations

Jan 23, 2020·MBio·Aaron S DhandaJulian A Guttman
May 10, 2020·Toxins·Franjo BanovićChristian Schwerk
May 5, 2020·Virulence·Caroline CharlierMarc Lecuit
Oct 7, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Aaron S DhandaJulian A Guttman
Oct 26, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Aaron S DhandaJulian A Guttman
Feb 16, 2021·Trends in Microbiology·Olivier DissonMarc Lecuit
Aug 27, 2021·Nature Reviews. Microbiology·Christina J Megli, Carolyn B Coyne
Oct 28, 2021·Molecular Microbiology·Keith IretonMazhar Hussain

❮ Previous
Next ❯

Methods Mentioned

BETA
pull-down
PCR2.1
PCR
flow cytometry
two-hybrid
pull down
pulled
Isothermal titration calorimetry

Software Mentioned

MathWorks
Elements
MATLAB
Coot
MicroManager
StepOnePlus
2000
HKL
Open Imaging
Origin

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie
Marc Lecuit
Infection and Immunity
Cristina FarallaAnna I Bakardjiev
Proceedings of the National Academy of Sciences of the United States of America
Cindy M QuezadaC Erec Stebbins
© 2022 Meta ULC. All rights reserved