PMID: 2117270Aug 1, 1990Paper

Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly

Proceedings of the National Academy of Sciences of the United States of America
G A DabiriF S Southwick

Abstract

Listeria monocytogenes is an intracellular parasite that can readily infect the macrophage-like cell line J774 and the kidney epithelial cell PtK2. After being ingested, the organism escapes from the phagolysosome into the host-cell cytoplasm. N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-phallacidin, a specific stain for actin filaments (F-actin), demonstrates that within 1 hr of initiation of infection, the bacteria become surrounded by host-cell cytoplasmic actin filaments. By 3 hr, long projections of F-actin begin to form at one end of the bacteria. These actin structures colocalize with the actin-bundling protein alpha-actinin as well as with tropomyosin. Microinjection of fluorescently labeled alpha-actinin in living cells demonstrates that the formation of these F-actin projections is associated with bacterial movement, actin filaments rapidly assembling behind the bacteria as they migrate through the cytoplasm. These F-actin tails attain lengths up to 40 microns. The movement of the bacteria through the cytoplasm is rapid, 0.12-1.46 microns/sec. Within 2 min of cytochalasin D (0.5 micrograms/ml) treatment, all bacterial intracellular movement stops, and additional bacteria-associated actin assembly is blocked. A nonmotile List...Continue Reading

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Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·J M SangerJ W Sanger
Dec 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F S Southwick, D L Purich
Sep 5, 2002·Cell Motility and the Cytoskeleton·Fangliang ZhangDaniel L Purich
Nov 27, 2002·Cell Motility and the Cytoskeleton·Frederick S SouthwickDaniel L Purich
Jan 1, 1993·Cell Motility and the Cytoskeleton·S H Zigmond
Jan 1, 1993·Cell Motility and the Cytoskeleton·K Imanaka-YoshidaJ W Sanger
Jan 1, 1994·Cell Motility and the Cytoskeleton·K L KingT S Moerland
Jan 1, 1994·Cell Motility and the Cytoskeleton·D NanavatiJ W Sanger
Jan 1, 1995·Cell Motility and the Cytoskeleton·V ZhukarevH Shuman
Nov 1, 1994·Research in Microbiology·M P ConteL Seganti
Feb 1, 1992·Current Opinion in Immunology·D A Portnoy
Feb 1, 1995·Current Opinion in Cell Biology·P Cossart
Aug 1, 1992·Trends in Cell Biology·J A Theriot, T J Mitchison
Feb 5, 2000·Biochimica Et Biophysica Acta·D J Olbris, J Herzfeld
Mar 4, 2000·Advanced Drug Delivery Reviews·C J Provoda, K D Lee
Jun 3, 1999·Cellular Signalling·T S HayesJ Gomez-Cambronero
Aug 24, 2000·Microbes and Infection·L Braun, P Cossart
Nov 13, 1999·Nature Cell Biology·O D WeinerH R Bourne
May 20, 2006·Nature Reviews. Microbiology·Mélanie HamonPascale Cossart
Mar 21, 1996·The New England Journal of Medicine·F S Southwick, D L Purich
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M F CarlierD Pantaloni
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May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·F S Southwick, D L Purich
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Oct 1, 1995·The Journal of Cell Biology·L P Cramer, T J Mitchison
Dec 1, 1992·Molecular Biology of the Cell·T J Mitchison

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