Listeriolysin O is essential for virulence of Listeria monocytogenes: direct evidence obtained by gene complementation.

Infection and Immunity
P CossartP Berche

Abstract

The role of listeriolysin O in the intracellular multiplication of Listeria monocytogenes and, therefore, its pathogenicity was questioned through a genetic complementation study. A nonhemolytic mutant was generated by inserting a single copy of transposon Tn917 in the bacterial chromosome. This insertion was localized by DNA sequence analysis in hlyA, the gene coding for listeriolysin O. As was another mutant that we previously characterized, this mutant was avirulent in the mouse. It was transformed with a plasmid carrying only hlyA, able to replicate in L. monocytogenes, and stably maintained in vitro and in vivo. The complemented strain displayed a hemolytic phenotype identical to that of the wild-type strain and was fully virulent, therefore attributing a crucial role to listeriolysin O in virulence and excluding the hypothesis of a polar effect of the transposon insertion on genes adjacent to hlyA and possibly involved in virulence.

References

Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Aug 16, 1976·Biochimica Et Biophysica Acta·D Prigent, J E Alouf
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 1, 1987·Plasmid·M F VicenteJ C Perez-Diaz
Apr 1, 1988·The Journal of Experimental Medicine·D A PortnoyD J Hinrichs
Aug 1, 1986·Journal of Bacteriology·M A Vandeyar, S A Zahler
Nov 1, 1986·Molecular & General Genetics : MGG·P Courvalin, C Carlier
May 1, 1972·The Journal of Experimental Medicine·F C Lane, E R Unanue
May 1, 1969·The Journal of Experimental Medicine·G B Mackaness
Jan 1, 1981·Cold Spring Harbor Symposia on Quantitative Biology·A E Franke, D B Clewell
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J YoungmanR Losick
Jan 1, 1983·Methods in Enzymology·J Messing
Jan 1, 1984·Medical Microbiology and Immunology·H Hof
Nov 1, 1957·Journal of Bacteriology·H F HASENCLEVER, W W KARAKAWA
Sep 1, 1962·The Journal of Experimental Medicine·G B MACKANESS

❮ Previous
Next ❯

Citations

Apr 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A PolancoM Meseguer
Jan 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A L Baetz, I V Wesley
Jan 1, 1996·Critical Reviews in Food Science and Nutrition·W E Hill
Jan 1, 1997·Critical Reviews in Microbiology·A K Bhunia
Sep 22, 1998·Nature Biotechnology·C Grillot-CourvalinP Courvalin
Mar 20, 2002·The Journal of Cell Biology·Shaynoor Dramsi, Pascale Cossart
May 14, 2004·Gene Therapy·R J CritchleyG Vassaux
Sep 1, 2004·The Journal of Cell Biology·Stéphanie SeveauPascale Cossart
Mar 24, 2006·FEMS Immunology and Medical Microbiology·Jarosław WiśniewskiJacek Bielecki
Aug 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mélanie Anne HamonPascale Cossart
Feb 7, 2008·Pathology International·Makoto Naito
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hideki HaraMasao Mitsuyama
Jun 23, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kohsuke TsuchiyaMasao Mitsuyama
Jan 5, 2011·Infection and Immunity·Glen P CarterDena Lyras
Feb 22, 2011·Biotechnology and Bioengineering·Chih-Hsiang ChangYun-Peng Chao
Mar 22, 2013·Biochemical Society Transactions·Leanne M Smith, Robin C May
Sep 24, 2013·PloS One·Olivia S SakhonJoao H F Pedra
Feb 11, 2014·BJOG : an International Journal of Obstetrics and Gynaecology·A JayaramS S Witkin
Aug 4, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mirosława SkupińskaMałgorzata Giel-Pietraszuk
Jan 21, 2017·Genes·Meredith SchroederAmanda E Brooks
Mar 20, 2020·International Journal of Molecular Sciences·Shuangqi FanJinding Chen
Jul 1, 1992·The Journal of Applied Bacteriology·S FitterC J Thomas
Jun 7, 1996·The Journal of Biological Chemistry·C Alvarez-DominguezP D Stahl
Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S E HamiltonJ T Harty

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.