Lithium chloride attenuates the abnormal osteogenic/adipogenic differentiation of bone marrow-derived mesenchymal stem cells obtained from rats with steroid-related osteonecrosis by activating the β-catenin pathway

International Journal of Molecular Medicine
Zefeng YuKunzheng Wang

Abstract

Steroid-related osteonecrosis of the femoral head (ONFH) may be a disease that results from the abnormal osteogenic/adipogenic differentiation of bone marrow-derived mesenchymal stem cells (BMMSCs). In the present study, we examined the possible use of lithium in an aim to reverse the abnormal osteogenic/adipogenic differentiation of BMMSCs isolated from rats with steroid-related ONFH (termed ONFH-BMMSCs). BMMSCs obtained from steroid‑related ONFH rat femurs were cultured with or without lithium chloride (LiCl). BMMSCs obtained from normal rat femurs were cultured as controls. LiCl significantly increased the expression of osteocalcin and Runx2 in the ONFH-BMMSCs during osteogenic induction. The mineralization of ONFH-BMMSCs following osteogenic induction was also enhanced. Furthermore, LiCl exerted anti-adipogenic effects on the ONFH-BMMSCs by inhibiting the expression of peroxisome proliferator-activated receptor γ (PPARγ) and fatty acid binding protein 4 (Fabp4) during adipogenic induction, and decreasing lipid droplet formation at the end of adipogenic induction. These effects of LiCl on the ONFH-BMMSCs were associated with an increased expression of β-catenin and a decreased expression of phosphorylated GSK-3β at Tyr-216, ...Continue Reading

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Citations

Jun 5, 2016·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Mahsan BanijamaliMaryam Shahhoseini
Jan 18, 2020·Journal of Cellular Physiology·Guan-Zhi LiuPei Yang
Oct 5, 2018·Bio-medical Materials and Engineering·Yue LuoPengDe Kang
Feb 8, 2019·Stem Cells International·Yi WangJie Tian
Sep 9, 2017·Metallomics : Integrated Biometal Science·Magali Roux, Anthony Dosseto

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Pro Plus
Image
SPSS
SDS

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