Lithium inhibits amplification or action of the maturation-promoting factor (MPF) in meiotic maturation of starfish oocytes

Experimental Cell Research
A Picard, M Doree

Abstract

Microinjection of LiCl reversibly inhibits hormone-induced meiotic maturation of starfish oocytes. Microinjection of NaCl (even in ouabain-treated oocytes) or KCl, or external application of LiCl have no such effect. Blockade of meiotic maturation by Li+ occurs even when microinjection is performed after the hormone dependent period has ended, that is the period during which the hormone must be present in the medium in order that meiosis can take place. Li+ microinjection prevents oocytes from meiosis reinitiation following transfer of cytoplasm taken from maturing oocytes, which contain a maturation-promoting factor (MPF). Cytoplasm taken from Li+-injected and hormone-treated oocytes does not trigger meiosis reinitiation when transferred in control immature oocytes. Intracellular pH does not change following LiCl microinjection. Simultaneous microinjection of either K+, Na+, or EGTA does not prevent Li+-dependent inhibition in oocytes.

References

Dec 8, 1978·Biochimica Et Biophysica Acta·L Beaugé
Aug 1, 1974·Experimental Cell Research·Y Hiramoto
Dec 1, 1973·Experimental Cell Research·T Kishimoto, H Kanatani
Aug 1, 1970·Experimental Cell Research·H Kanatani, Y Hiramoto
May 1, 1983·Experimental Cell Research·A Picard, M Dorée
Sep 1, 1936·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·C M Child

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