Lithium protects cartilage from cytokine-mediated degradation by reducing collagen-degrading MMP production via inhibition of the P38 mitogen-activated protein kinase pathway

Rheumatology
W HuiDavid A Young

Abstract

To determine the effects and mechanism of action of lithium chloride (LiCl) on cartilage destruction induced by the pro-inflammatory cytokines IL-1, IL-1 + oncostatin M and TNF-α. The release of collagen was assessed in bovine cartilage explant cultures, whereas collagenolytic activities (active and total) in conditioned culture supernatants were determined by bioassay. The expression and production of MMP from chondrocytes were analysed by real-time RT-PCR and ELISA. Signalling pathway analysis was performed using a phospho-antibody array and standard immunoblotting. LiCl, but not selective glycogen synthase kinase 3 (GSK-3) inhibitor compounds SB-415286 and TDZD-8, significantly decreased pro-inflammatory cytokine-induced collagen release from bovine cartilage via the down-regulation of collagenolytic activity. Furthermore, MMP-1 and MMP-13 expression was reduced in both bovine and human chondrocytes. Pathway analysis revealed that LiCl selectively inhibited activation of the p38 mitogen-activated protein kinase pathway; effects that were recapitulated by specific p38 pathway inhibition. This study demonstrates for the first time that LiCl can protect against cartilage damage induced by pro-inflammatory cytokines, and indicat...Continue Reading

References

Oct 4, 1995·Biochemical and Biophysical Research Communications·T E CawstonV Curry
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J D YorkP W Majerus
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·U Benbow, C E Brinckerhoff
Jul 1, 1997·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·M T Unlap, R S Jope
Dec 20, 1999·Analytical Biochemistry·P J KoshyT E Cawston
Feb 9, 2000·Trends in Pharmacological Sciences·R S Williams, A J Harwood
Oct 14, 2004·Clinical Orthopaedics and Related Research·Steven R Goldring, Mary B Goldring
Oct 21, 2004·Biochemical Society Transactions·R WilliamsA J Harwood
Apr 14, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Todd D Gould, Husseini K Manji
Nov 1, 2005·Mediators of Inflammation·Riina NieminenEeva Moilanen
Nov 17, 2005·Molecular and Cellular Biology·Victoria A BeardmoreJ Simon C Arthur
Nov 29, 2005·Joint, Bone, Spine : Revue Du Rhumatisme·Philippe Dieudé, François Cornélis
Dec 28, 2005·Current Medicinal Chemistry·Matthew A Brown
Apr 25, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Salvatore CuzzocreaChristoph Thiemermann
Dec 14, 2007·Expert Opinion on Therapeutic Targets·Andrew D RowanJennifer M Milner
Feb 9, 2008·Annals of the Rheumatic Diseases·Q ZhangD A Young
Feb 13, 2008·Cell·Matthew S Hayden, Sankar Ghosh
Aug 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joshua P FrederickJohn D York
Jan 31, 2009·Arthritis and Rheumatism·Mark C Genovese
May 2, 2009·The Journal of Biological Chemistry·Ian T StruewingCatherine D Mao
Nov 17, 2009·Cell and Tissue Research·Tim E Cawston, David A Young

❮ Previous
Next ❯

Citations

Mar 25, 2011·Journal of Agricultural and Food Chemistry·Yung-Chang LuGeorge Hsiao
Jan 5, 2011·Arthritis Research & Therapy·Jonathan B CatterallVirginia B Kraus
Nov 28, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Pongali B RaghavendraNarayanan Parameswaran
Oct 12, 2014·Biochemical and Biophysical Research Communications·Ye LiYong Hu
Jan 29, 2014·Arthritis & Rheumatology·Takeshi MinashimaThorsten Kirsch
Aug 31, 2013·Cellular & Molecular Biology Letters·Jing XuZhuo Yang
Jul 16, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Clare L ThompsonMartin M Knight
Jun 18, 2017·Journal of Biomaterials Applications·Siwei LiJulian R Jones
Oct 27, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Clare L ThompsonMartin M Knight
Oct 7, 2010·Nature Reviews. Rheumatology·Sarah Price
Feb 26, 2019·Bone & Joint Research·M McLeanN L Millar
Nov 16, 2019·Drug Metabolism Reviews·Dragos ApostuHorea Benea
Jan 1, 2017·Journal of Sol-gel Science and Technology·Anthony L B MaçonJulian R Jones
Oct 19, 2020·The European Journal of Neuroscience·Harry PantazopoulosSabina Berretta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.