Lithographic Microfabrication of a 16-Electrode Array on a Probe Tip for High Spatial Resolution Electrochemical Localization of Exocytosis

Analytical Chemistry
Joakim WigströmAnn-Sofie Cans

Abstract

We report the lithographic microfabrication of a movable thin film microelectrode array (MEA) probe consisting of 16 platinum band electrodes placed on top of a supporting borosilicate glass substrate. These 1.2 μm wide electrodes were tightly packed and positioned parallel in two opposite rows within a 20 μm × 25 μm square area and with a distance less than 10 μm from the edge of the glass substrate. We demonstrate the ability to control and place the probe in close proximity to the surface of adherent bovine chromaffin cells and to amperometrically record single exocytosis release events with high spatiotemporal resolution. The two-dimensional position of single exocytotic events occurring in the center gap area separating the two rows of MEA band electrodes and that were codetected by electrodes in both rows was determined by analysis of the fractional detection of catecholamine released between electrodes and exploiting random walk simulations. Hence, two-dimensional electrochemical imaging recording of exocytosis release between the electrodes within this area was achieved. Similarly, by modeling the current spikes codetected by parallel adjacent band electrodes positioned in the same electrode row, a one-dimensional imagi...Continue Reading

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Citations

Sep 4, 2017·Pflügers Archiv : European journal of physiology·Kevin D GillisValentina Carabelli
Sep 5, 2018·The Analyst·Qiushi HuangJin-Ming Lin
Jan 11, 2020·The Analyst·Pumidech Puthongkham, B Jill Venton
Mar 5, 2020·Lab on a Chip·Fumihiro SassaHiroaki Suzuki
Mar 11, 2020·Annual Review of Analytical Chemistry·Jacqueline D KeighronAnn-Sofie Cans
Sep 28, 2017·Pflügers Archiv : European journal of physiology·Hoda Fathali, Ann-Sofie Cans
Feb 12, 2019·Analytical Methods : Advancing Methods and Applications·Qun CaoB Jill Venton
Jun 6, 2017·Journal of Electroanalytical Chemistry·Christopher Gunderson, Bo Zhang
Dec 26, 2017·ACS Sensors·Jingjing ZhangHong-Yuan Chen
Jan 21, 2017·Analytical Chemistry·Lucas Armbrecht, Petra S Dittrich
Oct 27, 2018·Analytical Chemistry·Pieter E OomenAndrew G Ewing
Apr 24, 2018·Analytical Chemistry·Vera EßmannWolfgang Schuhmann
May 11, 2017·Analytical Chemistry·Huijie ZhangNicolas Plumeré

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