Little cigars and cigarillos harbor diverse bacterial communities that differ between the tobacco and the wrapper

PloS One
Suhana ChattopadhyayAmy R Sapkota

Abstract

Despite their potential importance with regard to infectious and chronic diseases among tobacco users, microbial constituents of tobacco products lack characterization. Specifically, to our knowledge, there are no data describing the bacterial diversity of little cigars or cigarillos. To address this knowledge gap, we tested four brands of little cigars and cigarillos. Tobacco and wrapper subsamples (n = 132) were separately subjected to DNA extraction, followed by PCR amplification of the V3V4 hypervariable region of the 16S rRNA gene, and sequencing using Illumina HiSeq. Sequences were analyzed using QIIME and Phyloseq implemented in R. We identified 2,681 operational taxonomic units across all products. Significant differences in alpha and beta diversity were observed between Swisher Sweets and Cheyenne products. Alpha and beta diversity was also significantly different between tobacco and wrapper subsamples within the same product. Beta diversity analyses of only tobacco samples identified no significant differences in the bacterial microbiota of different lots of the same products; however, the microbiota in the wrapper differed significantly across lots for all brands. Overall, Firmicutes were found to dominate in the wra...Continue Reading

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Citations

May 26, 2020·Applied Microbiology and Biotechnology·Leena MalayilAmy R Sapkota
Nov 12, 2019·Frontiers in Microbiology·Eoghan M SmythEmmanuel F Mongodin
Mar 12, 2021·Applied Microbiology and Biotechnology·Suhana ChattopadhyayAmy R Sapkota
Jun 11, 2021·Applied Microbiology and Biotechnology·A J Rivera, R E Tyx
Nov 19, 2021·Applied Microbiology and Biotechnology·Jianbin YeZhan Zhang

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Methods Mentioned

BETA
PCR

Software Mentioned

vegan package
VSEARCH
MetagenomeSeq
ANOSIM
DESeq2
Quantitative Insights Into Microbial Ecology ( QIIME
PANDAseq
QIIME
phyloseq
RStudio

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