Live-Cell High Content Screening in Drug Development

Methods in Molecular Biology
Milan EsnerMarc Bickle

Abstract

In the past decade, automated microscopy has become an important tool for the drug discovery and development process. The establishment of imaging modalities as screening tools depended on technological breakthroughs in the domain of automated microscopy and automated image analysis. These types of assays are often referred to as high content screening or high content analysis (HCS/HCA). The driving force to adopt imaging for drug development is the quantity and quality of cellular information that can be collected and the enhanced physiological relevance of cellular screening compared to biochemical screening. Most imaging in drug development is performed on fixed cells as this allows uncoupling the preparation of the cells from the acquisition of the images. Live-cell imaging is technically challenging, but is very useful for many aspects of the drug development pipeline such as kinetic studies of compound mode of action or to analyze the motion of cellular components. Most vendors of HCS microscopy systems offer the option of environmental chambers and onboard pipetting on their platforms. This reflects the wish and desire of many customers to have the ability to perform live-cell assays on their HCS automated microscopes. T...Continue Reading

Citations

Jan 18, 2020·Environmental Health Perspectives·Felicia Fei-Lei Chung, Zdenko Herceg
Mar 17, 2019·International Journal of Molecular Sciences·Gunjan PandeyJochen Gehrig
Jul 28, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Xi-Ting WangYu Li
Jan 29, 2020·Drug Discovery Today·Christian HausmannChristian Zoschke

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