Live cell imaging and systems biology

Wiley Interdisciplinary Reviews. Systems Biology and Medicine
Myong-Hee Sung, James G McNally

Abstract

Much of the experimental data used to construct mathematical models of molecular networks are derived from in vitro measurements. However, there is increasing evidence that in vitro measurements fail to capture both the complexity and the individuality found in single, living cells. These limitations can be overcome by live cell microscopy which is evolving to enable in vivo biochemistry. Here, we survey the current capabilities of live cell microscopy and illustrate how a number of different imaging approaches could be applied to analyze a specific molecular network. We argue that incorporation of such quantitative live-cell imaging methods is critical for the progress of systems biology.

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Citations

Jul 10, 2013·Nature Reviews. Genetics·Antoine CoulonDaniel R Larson
Jul 7, 2011·International Journal of Molecular Sciences·Sheng-Wen Chiu, Mark C Leake
Feb 26, 2016·Nature Methods·Myong-Hee SungGordon L Hager
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Jan 9, 2019·Frontiers in Immunology·Rachel H Nelson, David E Nelson
Sep 13, 2020·International Journal of Molecular Sciences·Stefan J RoobolJeroen Essers

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