Live-Cell Imaging at the Nanoscale with Bioconjugatable and Photoactivatable Fluorophores.

Bioconjugate Chemistry
Yang Zhang, Françisco M Raymo

Abstract

Optical diffraction fundamentally limits the spatial resolution of conventional fluorescence images to length scales that are, at least, 2 orders of magnitude longer than the dimensions of individual molecules. As a result, the development of innovative probes and imaging schemes to overcome diffraction is very much needed to enable the investigation of the fundamental factors regulating cellular functions at the molecular level. In this context, the chemical synthesis of molecular constructs with photoactivatable fluorescence and the ability to label subcellular components of live cells can have transformative implications. Indeed, the fluorescence of the resulting assemblies can be activated with spatiotemporal control, even in the intracellular environment, to permit the sequential localization of individual emissive labels with precision at the nanometer level and the gradual reconstruction of images with subdiffraction resolution. The implementation of these operating principles for subdiffraction imaging, however, is only possible if demanding photochemical and photophysical requirements to enable photoactivation and localization as well as stringent structural requisites to allow the covalent labeling of intracellular ta...Continue Reading

References

Jul 11, 1991·Nature·J A Theriot, T J Mitchison
Jul 14, 1998·Methods in Enzymology·T J MitchisonA Mallavarapu
May 22, 1989·Physical Review Letters·W E Moerner, L Kador
Aug 22, 2001·Bioorganic & Medicinal Chemistry Letters·K R GeeD J Kozlowski
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Dec 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexey Sharonov, Robin M Hochstrasser
Jul 21, 2007·Angewandte Chemie·J FöllingS W Hell
Jan 18, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jonas FöllingStefan W Hell
Jun 25, 2008·Journal of the American Chemical Society·Samuel J LordW E Moerner
Sep 17, 2008·Nature Methods·Jonas FöllingStefan W Hell
Nov 26, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Samuel J LordW E Moerner
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sri Rama Prasanna PavaniW E Moerner
Aug 12, 2009·Angewandte Chemie·Mike HeilemannMarkus Sauer
Sep 18, 2009·Chemistry : a European Journal·Vladimir N BelovStefan W Hell
Feb 1, 1995·Optics Letters·E Betzig
Oct 29, 2009·The Journal of Physical Chemistry. B·Samuel J LordW E Moerner
Dec 8, 2009·Journal of the American Chemical Society·Graham T DempseyXiaowei Zhuang
Feb 10, 2010·Journal of the American Chemical Society·David WartherMaurice Goeldner
Apr 15, 2010·Angewandte Chemie·Vladimir N BelovStefan W Hell
Aug 3, 2010·Bioorganic & Medicinal Chemistry·David PulitiMaurice Goeldner
Aug 10, 2010·Nature Methods·Richard WombacherMarkus Sauer
Oct 13, 2010·Journal of the American Chemical Society·Hsiao-lu D LeeW E Moerner
Dec 31, 2010·Nature Methods·Teresa KleinMarkus Sauer
May 10, 2011·Nature Methods·Sara A JonesXiaowei Zhuang
Jun 16, 2011·The Journal of Physical Chemistry. a·Valentine K JohnsYi Liao
Jul 2, 2011·Nature Protocols·Sebastian van de LindeMarkus Sauer
Sep 29, 2011·Angewandte Chemie·Laura M WysockiLuke D Lavis
Oct 27, 2011·ACS Chemical Biology·Sambashiva BanalaKai Johnsson
Nov 15, 2011·Current Opinion in Chemical Biology·Laura M Wysocki, Luke D Lavis
Jan 6, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kirill KolmakovStefan W Hell
Jan 19, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Wen-hong Li, Genhua Zheng
Mar 13, 2012·Annual Review of Physical Chemistry·Taekjip Ha, Philip Tinnefeld
Apr 24, 2012·Nano Letters·Alexander BenkeSuliana Manley
May 9, 2012·Annual Review of Biomedical Engineering·Travis J GouldJoerg Bewersdorf
May 15, 2012·Annual Review of Biophysics·Michael A ThompsonW E Moerner
Jun 15, 2012·Journal of the American Chemical Society·Tomonori KobayashiYasuteru Urano
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sang-Hee ShimXiaowei Zhuang
Aug 30, 2012·Angewandte Chemie·Raphaël LabruèreLudovic Jullien
Oct 30, 2012·Nature Methods·Joshua C VaughanXiaowei Zhuang

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Citations

Aug 7, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·E Abou NakadA Specht
Jun 2, 2021·Chemistry : a European Journal·Yang ZhangFrançisco M Raymo
Oct 31, 2020·Chemical Reviews·Roy WeinstainPetr Klán

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