Live Confocal Imaging as a Novel Tool to Assess Liver Quality: Insights From a Murine Model.

Transplantation
Benno CardiniRupert Oberhuber

Abstract

In an experimental murine liver clamping model, we aimed to investigate the efficacy of real-time confocal microscopy (RCM) in assessing viability of steatotic livers in comparison to standard assessment tools, including histopathological evaluation. C57Bl/6 mice were subjected to a methionine-choline-deficient diet causing nonalcoholic fatty liver disease or to Lieber DeCarli diet causing ethanol-induced liver injury. Untreated animals served as controls. Liver biopsies were analyzed following challenge with 45 min of warm ischemia time and either 4 h of reperfusion or 24 h of cold storage. Organ quality assessment was performed at defined time points by RCM, histological staining, measurement of serum alanine aminotransferase activity, and expression analyses of proinflammatory cytokines. Additionally, survival analysis was performed. Cold as well as warm ischemia time resulted in a significant decrease in cell viability when compared with naive livers as well as nonischemic-challenged steatotic livers (P < 0.05) as assessed by RCM. Furthermore, RCM revealed the actual cellular damage at early time points, while established methods including H&E-staining and serum transaminase profile failed. In a translational attempt, we de...Continue Reading

References

Apr 25, 2003·Experimental and Molecular Pathology·Constantino FondevilaJerzy W Kupiec-Weglinski
Jul 1, 2004·Alimentary Pharmacology & Therapeutics·V W-S WongJ J-Y Sung
Apr 1, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Carla S CoffinZu-hua Gao
Jan 30, 2008·Journal of Lipid Research·Mary E RinellaRichard M Green
Mar 28, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W Ray KimUNKNOWN Public Policy Committee of the American Association for the Study of Liver Disease
Nov 26, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Michelangelo FiorentinoAntonia D'Errico-Grigioni
Jun 29, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Austin L SpitzerJames D Perkins
Jul 25, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A E BraatUNKNOWN European Liver and Intestine Transplant Association (ELITA) and Eurotransplant Liver Intestine Advisory Committee (ELIAC)
Mar 2, 2013·Nature Protocols·Adeline BertolaBin Gao
Apr 23, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Muhammad Imtiaz AshrafMartin Hermann
May 7, 2014·Journal of Hepatology·Cornelia J VerhoevenLuc J W van der Laan
Apr 29, 2015·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Michael J J ChuAdam S J R Bartlett
Dec 20, 2015·Journal of Hepatology·Verena WieserHerbert Tilg
Feb 4, 2016·Expert Review of Gastroenterology & Hepatology·Balázs NemesSusumu Eguchi
Mar 23, 2018·Current Transplantation Reports·Christopher J E Watson, Ina Jochmans

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