Live Follow-Up of Enzymatic Reactions Inside the Cavities of Synthetic Giant Unilamellar Vesicles Equipped with Membrane Proteins Mimicking Cell Architecture

ACS Synthetic Biology
Martina GarniWolfgang Meier

Abstract

Compartmentalization of functional biological units, cells, and organelles serves as an inspiration for the development of biomimetic materials with unprecedented properties and applications in biosensing and medicine. Because of the complexity of cells, the design of ideal functional materials remains a challenge. An elegant strategy to obtain cell-like compartments as novel materials with biofunctionality is the combination of synthetic micrometer-sized giant unilamellar vesicles (GUVs) with biomolecules because it enables studying the behavior of biomolecules and processes within confined cavities. Here we introduce a functional cell-mimetic compartment formed by insertion of the model biopore bacterial membrane protein OmpF in thick synthetic membranes of an artificial GUV compartment that encloses-as a model-the oxidative enzyme horseradish peroxidase. In this manner, a simple and robust cell mimic is designed: the biopore serves as a gate that allows substrates to enter cavities of the GUVs, where they are converted into products by the encapsulated enzyme and then released in the environments of GUVs. Our bioequipped GUVs facilitate the control of specific catalytic reactions in confined microscale spaces mimicking cell ...Continue Reading

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Citations

Dec 21, 2019·Macromolecular Rapid Communications·Zhikun WangSongqing Hu
May 13, 2020·Physical Chemistry Chemical Physics : PCCP·Claire E MeyerCornelia G Palivan
Jun 12, 2019·Chembiochem : a European Journal of Chemical Biology·Matthias C HuberStefan M Schiller
May 27, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Luisa ZartnerCornelia G Palivan
May 30, 2020·Small·Edit BrodszkijBrigitte Städler
Nov 19, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Xiaomin QianBrigitte Städler
Dec 4, 2019·Nano Letters·Seong-Min JoKatharina Landfester

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