Live imaging of active fluorophore labelled Wnt proteins

FEBS Letters
Tatjana HolzerDietmar Gradl

Abstract

For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that this directed movement is essential for the secretion of active Wnt protein.

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Citations

Jan 30, 2013·FEBS Letters·Sonja HalbedlDietmar Gradl
Dec 2, 2015·Cellular and Molecular Life Sciences : CMLS·Felicitas PrölsMartin Scaal
Aug 8, 2012·Current Opinion in Genetics & Development·Jorge Soza-Ried, Amanda G Fisher
Oct 15, 2014·PloS One·Veronika WallkammDietmar Gradl
Jul 5, 2016·Oncogene·N SkalkaR Rosin-Arbesfeld
Jan 30, 2016·Journal of Cell Science·Eliana Stanganello, Steffen Scholpp
Apr 20, 2017·Journal of Cell Science·Hanna BergerAnnette Borchers
Jan 6, 2017·Journal of Biochemistry·Shinji TakadaYusuke Mii
Oct 16, 2018·Communications Biology·Ritsuko TakadaShinji Takada
Sep 16, 2017·Cellular and Molecular Life Sciences : CMLS·Lucy Brunt, Steffen Scholpp
Sep 27, 2018·Histochemistry and Cell Biology·Benjamin Mattes, Steffen Scholpp
May 17, 2019·Development·Daniel Routledge, Steffen Scholpp
Aug 4, 2021·PLoS Computational Biology·Adrián Aguirre-Tamaral, Isabel Guerrero

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