Live Imaging of Heart Injury in Larval Zebrafish Reveals a Multi-Stage Model of Neutrophil and Macrophage Migration.

Frontiers in Cell and Developmental Biology
A. KavehM. A. Denvir

Abstract

Neutrophils and macrophages are crucial effectors and modulators of repair and regeneration following myocardial infarction, but they cannot be easily observed in vivo in mammalian models. Hence many studies have utilized larval zebrafish injury models to examine neutrophils and macrophages in their tissue of interest. However, to date the migratory patterns and ontogeny of these recruited cells is unknown. In this study, we address this need by comparing our larval zebrafish model of cardiac injury to the archetypal tail fin injury model. Our in vivo imaging allowed comprehensive mapping of neutrophil and macrophage migration from primary hematopoietic sites, to the wound. Early following injury there is an acute phase of neutrophil recruitment that is followed by sustained macrophage recruitment. Both cell types are initially recruited locally and subsequently from distal sites, primarily the caudal hematopoietic tissue (CHT). Once liberated from the CHT, some neutrophils and macrophages enter circulation, but most use abluminal vascular endothelium to crawl through the larva. In both injury models the innate immune response resolves by reverse migration, with very little apoptosis or efferocytosis of neutrophils. Furthermore...Continue Reading

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Citations

Feb 17, 2021·Npj Regenerative Medicine·Luiza Farache Trajano, Nicola Smart
Jun 25, 2021·Cardiovascular Research·Katherine M Ross StewartMairi Brittan

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Methods Mentioned

BETA
transgenic
fluorescence microscopy
flow cytometry
RNAseq
FCS

Software Mentioned

MTrackJ
GraphPad Prism
FCS Express
GraphPad
Trackmate
Fiji
Imaris
ImageJ ( Fiji

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