Live imaging of mitosomes and hydrogenosomes by HaloTag technology.

PloS One
Eva MartincováPavel Dolezal

Abstract

Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively. The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.

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Citations

Aug 26, 2014·Current Opinion in Biotechnology·Arnab Mukherjee, Charles M Schroeder
May 15, 2015·Bioconjugate Chemistry·Christopher G EnglandWeibo Cai
Jun 10, 2015·Molecular and Cellular Biology·Eva MartincováPavel Doležal
Dec 15, 2015·Parasitology·Victoria Morin-Adeline, Jan Šlapeta
Oct 4, 2014·PloS One·Maria Francesca MossutoTiziana Anelli
Nov 22, 2019·PLoS Pathogens·Luboš Voleman, Pavel Doležal

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Methods Mentioned

BETA
proteomic
protein folding
CLIP
PCR
FRET
GTPases

Software Mentioned

HaloTag

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