Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters

Scientific Reports
Matteo ArdiniFrancesco De Angelis

Abstract

Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as a non-destructive and multiplexed technique, but intracellular SERS imaging still suffers from interfering background from endogenous components. Here we show the assembly of small colloidal SERS probes with Raman signal in the cell-silent window of 1800-2900 cm-1 for biorthogonal intracellular SERS imaging of dopamine that was undistinguishable from the endogenous cell background. By linking colloidal silver nanoparticles with alkyne-dopamine adducts, clusters are formed by 2-6 nanoparticles spaced by tight interparticle gaps that exhibited high electric field enhancement and strong SERS signals of alkyne and dopamines. Due to the cell-silent signals of the alkyne, intracellular in-vitro Raman imaging shows that the dopamines on the internalized clusters remain distinguishable across the cytoplasm with good spatial resolution. Our method can be a general-purpose method for real-time imaging of biomolecules, such as proteins, peptides, DNA and drugs.

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Citations

Feb 20, 2019·International Journal of Molecular Sciences·Sang Hun Lee, Bong-Hyun Jun
Jan 8, 2021·Bioconjugate Chemistry·Matteo ArdiniFrancesco Angelucci

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Methods Mentioned

BETA
electron
atomic force microscopy
fluorescence microscopy
imaging techniques
dynamic light scattering
light scattering
surface plasmon resonances
X-ray
electron microscopy
Scanning Electron Microscopy

Software Mentioned

ImageJ
Comsol Multiphysics®
GIMP

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