Live probiotic Bifidobacterium lactis bacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell culture.

Clinical and Experimental Immunology
K LindforsK Kaukinen

Abstract

Wheat gliadin induces severe intestinal symptoms and small-bowel mucosal damage in coeliac disease patients. At present, the only effective treatment for the disease is a strict life-long gluten-free diet. In this study we investigated whether probiotics Lactobacillus fermentum or Bifidobacterium lactis can inhibit the toxic effects of gliadin in intestinal cell culture conditions. The ability of live probiotics to inhibit peptic-tryptic digested gliadin-induced damage to human colon cells Caco-2 was evaluated by measuring epithelial permeability by transepithelial resistance, actin cytoskeleton arrangements by the extent of membrane ruffling and expression of tight junctional protein ZO-1. B. lactis inhibited the gliadin-induced increase dose-dependently in epithelial permeability, higher concentrations completely abolishing the gliadin-induced decrease in transepithelial resistance. The same bacterial strain also inhibited the formation of membrane ruffles in Caco-2 cells induced by gliadin administration. Furthermore, it also protected the tight junctions of Caco-2 cells against the effects of gliadin, as evinced by the pattern of ZO-1 expression. We conclude thus that live B. lactis bacteria can counteract directly the harm...Continue Reading

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References

Sep 12, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Felix HauschChaitan Khosla
Sep 28, 2002·Science·Lu ShanChaitan Khosla
Feb 10, 2004·Applied and Environmental Microbiology·Raffaella Di CagnoMarco Gobbetti
Mar 11, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jan-Michel Otte, Daniel K Podolsky
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marina NikulinaHubert Kolb
Sep 11, 2004·Immunity·Sophie HüeSophie Caillat-Zucman
Oct 11, 2005·Current Opinion in Immunology·Ludvig M Sollid, Bana Jabri
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Ludvig M Sollid, Chaitan Khosla
Nov 5, 2005·Applied and Environmental Microbiology·Trine Danø KlingbergBirgitte Bjørn Budde
Nov 16, 2005·Gastroenterology·Luigi MaiuriMarco Londei
Nov 29, 2005·Biochimica Et Biophysica Acta·Maria De AngelisMarco Gobbetti
Nov 30, 2005·Journal of Applied Microbiology·G RollánG F de Valdez
Mar 30, 2006·Journal of Agricultural and Food Chemistry·Teresa ZottaEugenio Parente
Apr 12, 2006·European Journal of Gastroenterology & Hepatology·David H DewarPaul J Ciclitira
Apr 20, 2006·Letters in Applied Microbiology·C L GerezG F de Valdez
Jun 15, 2006·European Journal of Gastroenterology & Hepatology·Peter D Howdle
Jun 23, 2007·Current Pharmaceutical Design·Annalisa IezziFrancesco Cipollone

❮ Previous
Next ❯

Citations

Aug 21, 2010·Inflammatory Bowel Diseases·Sa'ad Y Salim, Johan D Söderholm
Jun 8, 2012·Seminars in Immunopathology·Christopher S McAllister, Martin F Kagnoff
Apr 21, 2009·Applied and Environmental Microbiology·Raffaella Di CagnoMaria De Angelis
Aug 4, 2009·PloS One·Jane M NatividadElena F Verdú
Aug 23, 2012·World Journal of Gastroenterology : WJG·Yu-Kun ZhouZhi-Hua Liu
Aug 7, 2013·Nature Reviews. Gastroenterology & Hepatology·Katri KaukinenMarkku Mäki
Jun 28, 2012·Digestive Diseases and Sciences·Radu M Nanau, Manuela G Neuman
Nov 30, 2013·Applied Microbiology and Biotechnology·Diana Di GioiaBruno Biavati
Sep 16, 2014·Expert Opinion on Emerging Drugs·Peter D MooneyDavid S Sanders
Nov 27, 2009·Expert Opinion on Investigational Drugs·Luis Rodrigo
Aug 30, 2012·Expert Opinion on Biological Therapy·Marja-Leena LähdeahoMarkku Mäki
Jan 5, 2016·Digestive Diseases and Sciences·Giovanni MarascoDavide Festi
Oct 6, 2009·International Journal of Medical Microbiology : IJMM·Caroline Reiff, Denise Kelly
Jun 16, 2009·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Yan ZhongTing Han
Sep 29, 2015·Current Opinion in Pharmacology·Justin L McCarvilleElena F Verdu
Aug 16, 2015·Autoimmunity Reviews·Antonio Di SabatinoGino Roberto Corazza
Jan 18, 2011·Clinical and Experimental Immunology·T RauhavirtaK Lindfors
Dec 9, 2014·Gut Microbes·Heather J Galipeau, Elena F Verdu
Nov 5, 2010·Enzyme Research·Ivana CaputoCarla Esposito
Jun 10, 2015·Nature Reviews. Gastroenterology & Hepatology·Elena F VerduBana Jabri
May 25, 2012·Expert Opinion on Therapeutic Targets·Katri LindforsKatri Kaukinen
Oct 19, 2014·Gastroenterology Report·Natalia E CastilloDaniel A Leffler
Aug 16, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Joanna HarnettMargaret Rolfe
Jun 21, 2016·Journal of Pediatric Gastroenterology and Nutrition·Hilary JerichoStefano Guandalini
Apr 13, 2010·Expert Review of Clinical Immunology·Stefano Guandalini
Jun 13, 2014·Journal of Clinical Gastroenterology·Luis M Bustos FernandezFernando Man
Feb 1, 2017·Journal of Food Science·Jitendra KumarNar Singh Chauhan
Dec 19, 2014·Experimental Biology and Medicine·Martina BergerThomas Cesario
Sep 1, 2009·Therapeutic Advances in Gastroenterology·Christina A TennysonPeter H R Green
Jul 30, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Guoxian WeiEva J Helmerhorst
Sep 30, 2010·The American Journal of Gastroenterology·Maud PinierJean-Christophe Leroux
May 14, 2017·Applied and Environmental Microbiology·Ruggiero FrancavillaMarco Gobbetti
Jul 2, 2014·Clinical Microbiology Reviews·Luís Fernando de Sousa MoraesMaria do Carmo Gouveia Peluzio
Sep 8, 2010·Journal of Pediatric Gastroenterology and Nutrition·Erna SzikszBeáta Szebeni
Feb 12, 2019·Annals of Medicine·Giovanni Mario PesMaria Pina Dore
Apr 18, 2019·International Journal of Molecular Sciences·Antonella OrlandoFrancesco Russo

❮ Previous
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