Live Single-Cell Plant Hormone Analysis by Video-Mass Spectrometry

Plant & Cell Physiology
Takafumi ShimizuMitsunori Seo

Abstract

Studies have indicated that endogenous concentrations of plant hormones are regulated very locally within plants. To understand the mechanisms underlying hormone-mediated physiological processes, it is indispensable to know the exact hormone concentrations at cellular levels. In the present study, we established a system to determine levels of ABA and jasmonoyl-isoleucine (JA-Ile) from single cells. Samples taken from a cell of Vicia faba leaves using nano-electrospray ionization (ESI) tips under a microscope were directly introduced into mass spectrometers by infusion and subjected to tandem mass spectrometry (MS/MS) analysis. Stable isotope-labeled [D(6)]ABA or [(13)C(6)]JA-Ile was used as an internal standard to compensate ionization efficiencies, which determine the amount of ions introduced into mass spectrometers. We detected ABA and JA-Ile from single cells of water- and wound-stressed leaves, whereas they were almost undetectable in non-stressed single cells. The levels of ABA and JA-Ile found in the single-cell analysis were compared with levels found by analysis of purified extracts with liquid chromatography-tandem mass spectrometry (LC-MS/MS). These results demonstrated that stress-induced accumulation of ABA and JA...Continue Reading

References

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Citations

Oct 13, 2015·Current Biology : CB·Caspar ChaterAlistair M Hetherington
Jul 15, 2015·Plant & Cell Physiology·Akira Nagatani, Tetsuro Mimura
Feb 23, 2017·Annual Review of Plant Biology·Ondřej NovákKarin Ljung
Dec 9, 2017·Angewandte Chemie·Linwen Zhang, Akos Vertes
Mar 8, 2016·International Journal of Molecular Sciences·Hongyang Yu, Tengbo Huang
Nov 15, 2018·The Analyst·Kyle D DuncanIngela Lanekoff
Apr 20, 2017·The Plant Journal : for Cell and Molecular Biology·Dongmei LiYi Chen
Dec 2, 2020·International Journal of Molecular Sciences·Leonardo Perez de SouzaAlisdair Fernie
Jun 7, 2018·Analytical Chemistry·Ruichuan YinJulia Laskin

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