Liver-assist device with a microfluidics-based vascular bed in an animal model

Annals of Surgery
Wen-Ming HsuCraig Neville

Abstract

This study evaluates a novel liver-assist device platform with a microfluidics-modeled vascular network in a femoral arteriovenous shunt in rats. Liver-assist devices in clinical trials that use pumps to force separated plasma through packed beds of parenchymal cells exhibited significant necrosis with a negative impact on function. Microelectromechanical systems technology was used to design and fabricate a liver-assist device with a vascular network that supports a hepatic parenchymal compartment through a nanoporous membrane. Sixteen devices with rat primary hepatocytes and 12 with human HepG2/C3A cells were tested in athymic rats in a femoral arteriovenous shunt model. Several parenchymal tube configurations were evaluated for pressure profile and cell survival. The blood flow pattern and perfusion status of the devices was examined by laser Doppler scanning. Cell viability and serum protein secretion functions were assessed. Femoral arteriovenous shunt was successfully established in all animals. Blood flow was homogeneous through the vascular bed and replicated native flow patterns. Survival of seeded liver cells was highly dependent on parenchymal chamber pressures. The tube configuration that generated the lowest pressu...Continue Reading

References

Mar 1, 1979·Journal of Toxicology and Environmental Health·P O Seglen
May 1, 1994·Artificial Organs·N L SussmanJ H Kelly
Dec 29, 2000·Annals of Vascular Surgery·A CardonC J Doillon
Aug 30, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J W AllenS N Bhatia
Sep 29, 2001·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·G V MazariegosJ F Patzer
Dec 3, 2002·Tissue Engineering·Jared W Allen, Sangeeta N Bhatia
Mar 27, 2003·Journal of Hepato-biliary-pancreatic Surgery·Arno W TillesMehmet Toner
Nov 19, 2004·Biomedical Microdevices·Michael ShinJoseph P Vacanti
Feb 4, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Shintaro YagiShinji Uemoto
Mar 2, 2005·Tissue Engineering·Christina FidkowskiYadong Wang
Oct 20, 2005·Journal of Bioscience and Bioengineering·Jung-Keug Park, Doo-Hoon Lee
Jul 8, 2008·Biomedical Microdevices·Amedeo CarraroCraig Neville

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Citations

Aug 15, 2012·Biomechanics and Modeling in Mechanobiology·Douglas W EvansJessica L Sparks
Nov 9, 2011·Annals of Biomedical Engineering·Christopher MoraesShuichi Takayama
Sep 4, 2012·Journal of Biomechanical Engineering·Emma C MoranJessica L Sparks
Nov 2, 2011·Vascular Cell·Robert GauvinAli Khademhosseini
Jul 6, 2014·Seminars in Pediatric Surgery·Joseph P Vacanti, Katherine M Kulig
Jan 2, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Kenichiro NishiiJessica L Sparks
Nov 5, 2014·Expert Opinion on Biological Therapy·Martien van WenumRuurdtje Hoekstra
Jun 25, 2013·Current Opinion in Biotechnology·Basak E Uygun, Martin L Yarmush
Oct 31, 2012·Colloids and Surfaces. B, Biointerfaces·M MassimiC Cametti
Oct 5, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S J Karp, R B Mannon
Jun 25, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Dipali PatelAlexander Revzin
Dec 24, 2011·World Journal of Transplantation·Maurizio Salvadori
Aug 27, 2013·Expert Review of Medical Devices·Andrés Díaz LantadaJavier Urbieta Sotillo
Jul 28, 2013·Lab on a Chip·Sebastiaan J TrietschPaul Vulto

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