Liver cancer cells are sensitive to Lanatoside C induced cell death independent of their PTEN status

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Irem DurmazRengul Cetin-Atalay

Abstract

Hepatocellular carcinoma is the second deadliest cancer with limited treatment options. Loss of PTEN causes the P13K/Akt pathway to be hyperactive which contributes to cell survival and resistance to therapeutics in various cancers, including the liver cancer. Hence molecules targeting this pathway present good therapeutic strategies for liver cancer. It was previously reported that Cardiac glycosides possessed antitumor activity by inducing apoptosis of multiple cancer cells through oxidative stress. However, whether Cardiac glycoside Lanatoside C can induce oxidative stress in liver cancer cells and induce cell death both in vitro and in vivo remains unknown. Cell viability was measured by SRB assay. Cell death analysis was investigated by propidium iodide staining with flow cytometry and PARP cleavage. DCFH-DA staining and cytometry were used for intracellular ROS measurement. Protein levels were analyzed by western blot analysis. Antitumor activity was investigated on mice xenografts in vivo. In this study, we found that Cardiac glycosides, particularly Lanatoside C from Digitalis ferruginea could significantly inhibit PTEN protein adequate Huh7 and PTEN deficient Mahlavu human liver cancer cell proliferation by the inducti...Continue Reading

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Citations

Nov 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Naira Fernanda Zanchett SchneiderMarc Diederich
Sep 27, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Burcu ÇalışkanErden Banoglu
Jul 18, 2020·Scientific Reports·Irem Durmaz SahinRengul Cetin-Atalay
Oct 7, 2016·MedChemComm·Deniz Cansen KahramanRengul Cetin-Atalay
Jun 18, 2017·Scientific Reports·Qi WuQiang Liu
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dhanasekhar ReddyPreetam Ghosh
Aug 14, 2020·ACS Combinatorial Science·Jude I Ayogu, Amaechi S Odoh

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