PMID: 9428789Jan 15, 1998Paper

Liver-enriched transcription factors, HNF-1, HNF-3, and C/EBP, are major contributors to the strong activity of the chicken CYP2H1 promoter in chick embryo hepatocytes

DNA and Cell Biology
S C Dogra, B K May

Abstract

Chicken CYP2H1 promoter constructs express strongly in chick embryo hepatocytes at a level comparable with that of Rous sarcoma viral promoter. We have identified the transcription factors responsible for the active CYP2H1 promoter. Binding sites for transcription factors were located within the first 160 bp of promoter sequence using promoter deletion experiments and DNase I footprint analysis. Sequence analysis revealed characteristic sites for the liver-enriched transcription factors of the HNF-1, HNF-3, and C/EBP families and for the ubiquitous factor, USF. Protein binding to these sites was established by gel mobility shift assays. Mutagenesis and transient transfection experiments demonstrated that these sites, in combination, were responsible for the strong promoter activity with a substantial contribution from HNF-1 and HNF-3. The promoter was also active in mammalian HepG2 and COS-1 cell lines where expression was dependent on the identified transcription factor binding sites but promoter activity in the HeLa cells was low. Transactivation experiments revealed that promoter expression could be activated through the appropriate binding sites by exogenously expressed rat HNF-1alpha or HNF-1beta, rat HNF-3alpha or HNF-3be...Continue Reading

References

Aug 11, 1992·Nucleic Acids Research·C F CalkhovenJ Wijnholds
Feb 1, 1992·The Biochemical Journal·D J Waxman, L Azaroff
Dec 1, 1991·Current Opinion in Cell Biology·V De Simone, R Cortese
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K G XanthopoulosJ E Darnell
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K S ZaretC M DiPersio
Jan 1, 1990·Pharmacology & Therapeutics·D E Ryan, W Levin
Jul 1, 1988·Genes & Development·W H LandschulzS L McKnight
Jan 1, 1986·CRC Critical Reviews in Biochemistry·M Adesnik, M Atchison
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·L PrabhuG Padmanaban
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A CveklJ Piatigorsky
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·R S GoomerG M Edelman
Jul 1, 1993·Biological Chemistry Hoppe-Seyler·A HörleinT Igo-Kemenes
Apr 1, 1993·Current Opinion in Genetics & Development·L P Hansen, G R Crabtree
Apr 19, 1996·The Journal of Biological Chemistry·P HonkakoskiM Negishi
Jun 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J P WhitlockH Li
Aug 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F J Gonzalez, Y H Lee
Aug 1, 1996·DNA and Cell Biology·Y Park, B Kemper
Nov 1, 1996·Molecular and Cellular Biology·U Samadani, R H Costa

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Citations

Sep 12, 2001·The International Journal of Biochemistry & Cell Biology·B P DavidsonB K May
Jul 17, 2012·Fish & Shellfish Immunology·Wickramaarachchige Don Niroshana WickramaarachchiJehee Lee
Mar 11, 1998·Clinical and Experimental Pharmacology & Physiology·S C DograB K May
Dec 6, 2003·Pharmacological Reviews·Christoph Handschin, Urs A Meyer
Mar 21, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Joyce K DivineTheodore C Simon
Oct 15, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher W RowleyTheodore C Simon

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