Liver hepcidin and stainable iron expression in biliary atresia

Pediatric Research
Ying-Hsien HuangJiin-Haur Chuang

Abstract

Hepcidin is a proposed mammalian host defense peptide that was identified on the basis of its antimicrobial activity, but it was later shown to be a crucial regulator of iron homeostasis and a mediator of the anemia of chronic inflammation. Hepcidin and stainable iron expression in biliary atresia (BA) were investigated in this study. Fresh liver tissues were obtained from 10 patients in the early stage of BA when they underwent Kasai's procedure, 9 in the late stage of BA when they received liver transplantation and 5 controls receiving liver resection for benign lesions other than cholestasis or fibrosis. Real-time quantitative reverse-transcription PCR (QRT-PCR), immunohistochemical staining and ELISA were performed to gauge hepcidin mRNA and protein expression in liver and plasma. Archival liver specimens from patients in the early and late stages of BA were treated with Perls' acid ferrocyanide technique for hepatic stainable iron. The results demonstrated that liver hepcidin mRNA expression was 100-fold lower in late-stage BA than in the early stage by QRT-PCR. Significantly weaker liver hepcidin immunostaining and lower plasma hepcidin levels were found in late-stage BA than in the early stage. There was also significant...Continue Reading

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Citations

Oct 16, 2015·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Murat CakirSelim Demir
Sep 4, 2016·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Ying-Hsien HuangJiin-Haur Chuang
Aug 5, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ying-Hsien HuangChao-Long Chen
May 25, 2013·World Journal of Gastroenterology : WJG·Qiu-Ming WangZhen Liu

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